Formulation and In Vitro Evaluation of Casein Nanoparticles as Carrier for Celecoxib.

Madan, JR; Ansari, IN; Dua, K; Awasthi, R

Formulation and In Vitro Evaluation of Casein Nanoparticles as Carrier for Celecoxib.

Madan, JR Ansari, IN Dua, K

Awasthi, R

Permalink

Publisher:: Maad Rayan Publishing Company
Publication Type:: Journal Article
Citation:: Advanced pharmaceutical bulletin, 2020, 10, (3), pp. 408-417
Issue Date:: 2020-07

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download full textAdobe PDF (1.65 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Madan, JR
dc.contributor.author	Ansari, IN
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.contributor.author	Awasthi, R
dc.date.accessioned	2020-11-24T01:24:50Z
dc.date.available	2020-02-03
dc.date.available	2020-11-24T01:24:50Z
dc.date.issued	2020-07
dc.identifier.citation	Advanced pharmaceutical bulletin, 2020, 10, (3), pp. 408-417
dc.identifier.issn	2228-5881
dc.identifier.issn	2251-7308
dc.identifier.uri	http://hdl.handle.net/10453/144284
dc.description.abstract	Purpose: The objective of this work was to formulate casein (CAS) nanocarriers for the dissolution enhancement of poorly water soluble drug celecoxib (CLXB). Methods: The CLXB loaded CAS nanocarriers viz., nanoparticles, reassembled CAS micelles and nanocapsules were prepared using sodium caseinate (SOD-CAS) as a carrier to enhance the solubility of CLXB. The prepared formulations were characterized for particle size, polydispersity index, zeta potential, percentage entrapment efficiency, and surface morphology for the selection of best formulation. Fourier transform infrared spectroscopy, differential scanning calorimetry and X-ray powder diffraction study was used to for the confirmation of encapsulation of CLXB. Further,in vitro drug dissolution, ex-vivo permeation studies on chicken ileum and stability studies were carried out. Results: The CLXB loaded casein nanoparticles (CNP) (batch A2) showed a particle size diameter 216.1 nm, polydispersity index 0.422 with percentage entrapment efficiency of 90.71% and zeta potential of -24.6 mV. Scanning electron microscopy of suspension confirmed globular shape of CNP. Thein vitro release data of optimized batch followed non Fickian diffusion mechanism. The ex vivo permeation studies on chicken ileum of CLXB loaded CNP showed permeation through mucous membrane as compared to pure CLXB. The apparent permeability of best selected freeze dried CLXB loaded CNP (batch A2) was higher and gradually increased from 0.90 mg/cm2 after 10 min to a maximum of 1.95 mg/cm2 over the subsequent 90 min. A higher permeation was recorded at each time point than that of the pure CLXB. Conclusion: The study explored the potential of CAS as a carrier for solubility enhancement of poorly water soluble drugs.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Maad Rayan Publishing Company
dc.relation.ispartof	Advanced pharmaceutical bulletin
dc.relation.isbasedon	10.34172/apb.2020.049
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Formulation and In Vitro Evaluation of Casein Nanoparticles as Carrier for Celecoxib.
dc.type	Journal Article
utslib.citation.volume	10
utslib.location.activity	Iran
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health/GSH.Pharmacy
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2020-11-24T01:24:43Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	10
utslib.citation.issue	3

Abstract:

Purpose: The objective of this work was to formulate casein (CAS) nanocarriers for the dissolution enhancement of poorly water soluble drug celecoxib (CLXB). Methods: The CLXB loaded CAS nanocarriers viz., nanoparticles, reassembled CAS micelles and nanocapsules were prepared using sodium caseinate (SOD-CAS) as a carrier to enhance the solubility of CLXB. The prepared formulations were characterized for particle size, polydispersity index, zeta potential, percentage entrapment efficiency, and surface morphology for the selection of best formulation. Fourier transform infrared spectroscopy, differential scanning calorimetry and X-ray powder diffraction study was used to for the confirmation of encapsulation of CLXB. Further,in vitro drug dissolution, ex-vivo permeation studies on chicken ileum and stability studies were carried out. Results: The CLXB loaded casein nanoparticles (CNP) (batch A2) showed a particle size diameter 216.1 nm, polydispersity index 0.422 with percentage entrapment efficiency of 90.71% and zeta potential of -24.6 mV. Scanning electron microscopy of suspension confirmed globular shape of CNP. Thein vitro release data of optimized batch followed non Fickian diffusion mechanism. The ex vivo permeation studies on chicken ileum of CLXB loaded CNP showed permeation through mucous membrane as compared to pure CLXB. The apparent permeability of best selected freeze dried CLXB loaded CNP (batch A2) was higher and gradually increased from 0.90 mg/cm2 after 10 min to a maximum of 1.95 mg/cm2 over the subsequent 90 min. A higher permeation was recorded at each time point than that of the pure CLXB. Conclusion: The study explored the potential of CAS as a carrier for solubility enhancement of poorly water soluble drugs.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/144284