Development of a novel HPTLC fingerprint method for simultaneous estimation of berberine and rutin in medicinal plants and their pharmaceutical preparations followed by its application in antioxidant assay

Satija, S; Tambuwala, MM; Pabreja, K; Bakshi, HA; Chellappan, DK; Aljabali, AA; Nammi, S; Singh, TG; Dureja, H; Gupta, G; Dua, K; Mehta, M; Garg, M

Development of a novel HPTLC fingerprint method for simultaneous estimation of berberine and rutin in medicinal plants and their pharmaceutical preparations followed by its application in antioxidant assay

Satija, S

Tambuwala, MM Pabreja, K Bakshi, HA Chellappan, DK Aljabali, AA Nammi, S Singh, TG Dureja, H Gupta, G Dua, K

Mehta, M Garg, M

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Publisher:: SPRINGER HEIDELBERG
Publication Type:: Journal Article
Citation:: Journal of Planar Chromatography - Modern TLC, 2020, 33, (3), pp. 313-319
Issue Date:: 2020-06-01

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Field	Value	Language
dc.contributor.author	Satija, S https://orcid.org/0000-0003-4702-6534
dc.contributor.author	Tambuwala, MM
dc.contributor.author	Pabreja, K
dc.contributor.author	Bakshi, HA
dc.contributor.author	Chellappan, DK
dc.contributor.author	Aljabali, AA
dc.contributor.author	Nammi, S
dc.contributor.author	Singh, TG
dc.contributor.author	Dureja, H
dc.contributor.author	Gupta, G
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.contributor.author	Mehta, M
dc.contributor.author	Garg, M
dc.date.accessioned	2021-05-16T23:36:40Z
dc.date.available	2021-05-16T23:36:40Z
dc.date.issued	2020-06-01
dc.identifier.citation	Journal of Planar Chromatography - Modern TLC, 2020, 33, (3), pp. 313-319
dc.identifier.issn	0933-4173
dc.identifier.issn	1789-0993
dc.identifier.uri	http://hdl.handle.net/10453/148924
dc.description.abstract	The present study was designed to develop and validate a high-performance thin-layer chromatography (HPTLC) system for the simultaneous quantitative determination of berberine and rutin in Tinospora cordifolia extract and their pharmaceutical preparations. Chromatographic development was done using a blend of n-hexane, ethyl acetate, glacial acetic acid and methanol (10:1.1:1.1:2.5, v/v) as the mobile phase. Detection was completed densitometrically at 254 nm. The R estimation of berberine and rutin was observed to be 0.67 ± 0.02 and 0.47 ± 0.02, respectively. The developed HPTLC method was validated according to ICH guidelines; the method was specific, linear and accurate and can be used to determine berberine and rutin in marketed herbal preparations. The Tinospora cordifolia plant extract was further evaluated for antioxidant activity using HPTLC, and berberine was found to be more active than rutin during DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity. The method was found simple, rapid, accurate, specific and robust for the analysis of berberine and rutin in crude drug using the same method. [Figure not available: see fulltext.] F
dc.language	English
dc.publisher	SPRINGER HEIDELBERG
dc.relation.ispartof	Journal of Planar Chromatography - Modern TLC
dc.relation.isbasedon	10.1007/s00764-020-00035-y
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0301 Analytical Chemistry
dc.subject.classification	Analytical Chemistry
dc.title	Development of a novel HPTLC fingerprint method for simultaneous estimation of berberine and rutin in medicinal plants and their pharmaceutical preparations followed by its application in antioxidant assay
dc.type	Journal Article
utslib.citation.volume	33
utslib.for	0301 Analytical Chemistry
utslib.for	0301 Analytical Chemistry
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health/GSH.Pharmacy
utslib.copyright.status	closed_access	*
dc.date.updated	2021-05-16T23:36:39Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	33
utslib.citation.issue	3

Abstract:

The present study was designed to develop and validate a high-performance thin-layer chromatography (HPTLC) system for the simultaneous quantitative determination of berberine and rutin in Tinospora cordifolia extract and their pharmaceutical preparations. Chromatographic development was done using a blend of n-hexane, ethyl acetate, glacial acetic acid and methanol (10:1.1:1.1:2.5, v/v) as the mobile phase. Detection was completed densitometrically at 254 nm. The R estimation of berberine and rutin was observed to be 0.67 ± 0.02 and 0.47 ± 0.02, respectively. The developed HPTLC method was validated according to ICH guidelines; the method was specific, linear and accurate and can be used to determine berberine and rutin in marketed herbal preparations. The Tinospora cordifolia plant extract was further evaluated for antioxidant activity using HPTLC, and berberine was found to be more active than rutin during DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity. The method was found simple, rapid, accurate, specific and robust for the analysis of berberine and rutin in crude drug using the same method. [Figure not available: see fulltext.] F

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http://hdl.handle.net/10453/148924