Multiple holins contribute to extracellular DNA release in Pseudomonas aeruginosa biofilms.

Hynen, AL; Lazenby, JJ; Savva, GM; McCaughey, LC; Turnbull, L; Nolan, LM; Whitchurch, CB

Multiple holins contribute to extracellular DNA release in Pseudomonas aeruginosa biofilms.

Hynen, AL Lazenby, JJ Savva, GM McCaughey, LC Turnbull, L Nolan, LM Whitchurch, CB

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Publisher:: MICROBIOLOGY SOC
Publication Type:: Journal Article
Citation:: Microbiology, 2021, 167, (2)
Issue Date:: 2021-02-01

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Field	Value	Language
dc.contributor.author	Hynen, AL
dc.contributor.author	Lazenby, JJ
dc.contributor.author	Savva, GM
dc.contributor.author	McCaughey, LC
dc.contributor.author	Turnbull, L
dc.contributor.author	Nolan, LM
dc.contributor.author	Whitchurch, CB
dc.date.accessioned	2022-01-07T00:36:55Z
dc.date.available	2022-01-07T00:36:55Z
dc.date.issued	2021-02-01
dc.identifier.citation	Microbiology, 2021, 167, (2)
dc.identifier.issn	1350-0872
dc.identifier.issn	1465-2080
dc.identifier.uri	http://hdl.handle.net/10453/152796
dc.description.abstract	Bacterial biofilms are composed of aggregates of cells encased within a matrix of extracellular polymeric substances (EPS). One key EPS component is extracellular DNA (eDNA), which acts as a 'glue', facilitating cell-cell and cell-substratum interactions. We have previously demonstrated that eDNA is produced in Pseudomonas aeruginosa biofilms via explosive cell lysis. This phenomenon involves a subset of the bacterial population explosively lysing, due to peptidoglycan degradation by the endolysin Lys. Here we demonstrate that in P. aeruginosa three holins, AlpB, CidA and Hol, are involved in Lys-mediated eDNA release within both submerged (hydrated) and interstitial (actively expanding) biofilms, albeit to different extents, depending upon the type of biofilm and the stage of biofilm development. We also demonstrate that eDNA release events determine the sites at which cells begin to cluster to initiate microcolony formation during the early stages of submerged biofilm development. Furthermore, our results show that sustained release of eDNA is required for cell cluster consolidation and subsequent microcolony development in submerged biofilms. Overall, this study adds to our understanding of how eDNA release is controlled temporally and spatially within P. aeruginosa biofilms.
dc.format	Print
dc.language	eng
dc.publisher	MICROBIOLOGY SOC
dc.relation	Wellcome Trust106064/Z/14/Z
dc.relation	Biotechnology and Biological Sciences Research CouncilBBS/E/F/000PR10349
dc.relation.ispartof	Microbiology
dc.relation.isbasedon	10.1099/mic.0.000990
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	Microbiology
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	Bacteriolysis
dc.subject.mesh	Biofilms
dc.subject.mesh	DNA, Bacterial
dc.subject.mesh	Endopeptidases
dc.subject.mesh	Extracellular Polymeric Substance Matrix
dc.subject.mesh	Mutation
dc.subject.mesh	Pseudomonas aeruginosa
dc.subject.mesh	Biofilms
dc.subject.mesh	Pseudomonas aeruginosa
dc.subject.mesh	Endopeptidases
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	DNA, Bacterial
dc.subject.mesh	Bacteriolysis
dc.subject.mesh	Mutation
dc.subject.mesh	Extracellular Polymeric Substance Matrix
dc.title	Multiple holins contribute to extracellular DNA release in Pseudomonas aeruginosa biofilms.
dc.type	Journal Article
utslib.citation.volume	167
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access	*
dc.date.updated	2022-01-07T00:36:49Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	167
utslib.citation.issue	2

Abstract:

Bacterial biofilms are composed of aggregates of cells encased within a matrix of extracellular polymeric substances (EPS). One key EPS component is extracellular DNA (eDNA), which acts as a 'glue', facilitating cell-cell and cell-substratum interactions. We have previously demonstrated that eDNA is produced in Pseudomonas aeruginosa biofilms via explosive cell lysis. This phenomenon involves a subset of the bacterial population explosively lysing, due to peptidoglycan degradation by the endolysin Lys. Here we demonstrate that in P. aeruginosa three holins, AlpB, CidA and Hol, are involved in Lys-mediated eDNA release within both submerged (hydrated) and interstitial (actively expanding) biofilms, albeit to different extents, depending upon the type of biofilm and the stage of biofilm development. We also demonstrate that eDNA release events determine the sites at which cells begin to cluster to initiate microcolony formation during the early stages of submerged biofilm development. Furthermore, our results show that sustained release of eDNA is required for cell cluster consolidation and subsequent microcolony development in submerged biofilms. Overall, this study adds to our understanding of how eDNA release is controlled temporally and spatially within P. aeruginosa biofilms.

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http://hdl.handle.net/10453/152796