IP10 is a predictor of successful vaccine protection against paratuberculosis infection in sheep.

Pooley, HB; Panag, G; Plain, KM; de Silva, K; Begg, DJ; Whittington, RJ; Purdie, AC

IP10 is a predictor of successful vaccine protection against paratuberculosis infection in sheep.

Pooley, HB Panag, G Plain, KM de Silva, K

Begg, DJ Whittington, RJ Purdie, AC

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Publisher:: ELSEVIER SCI LTD
Publication Type:: Journal Article
Citation:: Vaccine, 2023, 41, (1), pp. 274-283
Issue Date:: 2023-01-04

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Field	Value	Language
dc.contributor.author	Pooley, HB
dc.contributor.author	Panag, G
dc.contributor.author	Plain, KM
dc.contributor.author	de Silva, K https://orcid.org/0000-0001-5973-5077
dc.contributor.author	Begg, DJ
dc.contributor.author	Whittington, RJ
dc.contributor.author	Purdie, AC
dc.date.accessioned	2024-05-10T19:35:42Z
dc.date.available	2022-11-13
dc.date.available	2024-05-10T19:35:42Z
dc.date.issued	2023-01-04
dc.identifier.citation	Vaccine, 2023, 41, (1), pp. 274-283
dc.identifier.issn	0264-410X
dc.identifier.issn	1873-2518
dc.identifier.uri	http://hdl.handle.net/10453/178831
dc.description.abstract	The cell mediated immune response and ability of immune cells to migrate to the site of infection are both key aspects of protection against many pathogens. Mycobacterium avium subsp. paratuberculosis (MAP) is an intracellular pathogen and the causative agent of paratuberculosis, a chronic wasting disease of ruminants. Current commercial vaccines for paratuberculosis reduce the occurrence of clinical disease but not all animals are protected from infection. Therefore, there is a need to understand the immune responses triggered by these vaccines at the site of infection, in circulating immune cells and their relationships to vaccine-mediated protection. The magnitude and location of gene expression related to the cell mediated immune response and cellular migration were studied in the ileum of sheep. In addition, longitudinal IP10 (also known as IP10) secretion by circulating immune cells was examined in the same sheep. Animals were grouped based on vaccination status (vaccinated vs non-vaccinated) and MAP exposure (experimentally exposed vs unexposed). Vaccination of unexposed sheep increased the expression of IP10, CCL5 and COR1c. Sheep that were successfully protected by vaccination (uninfected following experimental exposure) had significantly reduced expression of IP10 in the ileum at 12 months post exposure compared to vaccine non-responders (those that became infected) and non-vaccinated infected sheep. Successfully protected sheep also had significantly increased secretion of IP10 in in vitro stimulated immune cells from whole blood compared to vaccine non responders at 4 months post exposure. Therefore, the IP10 recall response has the potential to be used as marker for infection status in vaccinated sheep and could be a biomarker for a DIVA test in sheep.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ELSEVIER SCI LTD
dc.relation.ispartof	Vaccine
dc.relation.isbasedon	10.1016/j.vaccine.2022.11.018
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	06 Biological Sciences, 07 Agricultural and Veterinary Sciences, 11 Medical and Health Sciences
dc.subject.classification	Virology
dc.subject.classification	32 Biomedical and clinical sciences
dc.subject.classification	42 Health sciences
dc.subject.mesh	Sheep
dc.subject.mesh	Animals
dc.subject.mesh	Paratuberculosis
dc.subject.mesh	Chemokine CXCL10
dc.subject.mesh	Bacterial Vaccines
dc.subject.mesh	Antibodies, Bacterial
dc.subject.mesh	Mycobacterium avium subsp. paratuberculosis
dc.subject.mesh	Sheep Diseases
dc.subject.mesh	Animals
dc.subject.mesh	Sheep
dc.subject.mesh	Paratuberculosis
dc.subject.mesh	Sheep Diseases
dc.subject.mesh	Bacterial Vaccines
dc.subject.mesh	Antibodies, Bacterial
dc.subject.mesh	Mycobacterium avium subsp. paratuberculosis
dc.subject.mesh	Chemokine CXCL10
dc.subject.mesh	Sheep
dc.subject.mesh	Animals
dc.subject.mesh	Paratuberculosis
dc.subject.mesh	Chemokine CXCL10
dc.subject.mesh	Bacterial Vaccines
dc.subject.mesh	Antibodies, Bacterial
dc.subject.mesh	Mycobacterium avium subsp. paratuberculosis
dc.subject.mesh	Sheep Diseases
dc.title	IP10 is a predictor of successful vaccine protection against paratuberculosis infection in sheep.
dc.type	Journal Article
utslib.citation.volume	41
utslib.location.activity	Netherlands
utslib.for	06 Biological Sciences
utslib.for	07 Agricultural and Veterinary Sciences
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Provost
utslib.copyright.status	open_access	*
dc.date.updated	2024-05-10T19:35:40Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	41
utslib.citation.issue	1

Abstract:

The cell mediated immune response and ability of immune cells to migrate to the site of infection are both key aspects of protection against many pathogens. Mycobacterium avium subsp. paratuberculosis (MAP) is an intracellular pathogen and the causative agent of paratuberculosis, a chronic wasting disease of ruminants. Current commercial vaccines for paratuberculosis reduce the occurrence of clinical disease but not all animals are protected from infection. Therefore, there is a need to understand the immune responses triggered by these vaccines at the site of infection, in circulating immune cells and their relationships to vaccine-mediated protection. The magnitude and location of gene expression related to the cell mediated immune response and cellular migration were studied in the ileum of sheep. In addition, longitudinal IP10 (also known as IP10) secretion by circulating immune cells was examined in the same sheep. Animals were grouped based on vaccination status (vaccinated vs non-vaccinated) and MAP exposure (experimentally exposed vs unexposed). Vaccination of unexposed sheep increased the expression of IP10, CCL5 and COR1c. Sheep that were successfully protected by vaccination (uninfected following experimental exposure) had significantly reduced expression of IP10 in the ileum at 12 months post exposure compared to vaccine non-responders (those that became infected) and non-vaccinated infected sheep. Successfully protected sheep also had significantly increased secretion of IP10 in in vitro stimulated immune cells from whole blood compared to vaccine non responders at 4 months post exposure. Therefore, the IP10 recall response has the potential to be used as marker for infection status in vaccinated sheep and could be a biomarker for a DIVA test in sheep.

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http://hdl.handle.net/10453/178831