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Sarah J. Glastras, Hui Chen, Michael Tsang, Rachel Teh, Rachel T. McGrath, Amgad Zaky, Jason Chen, Muh Geot Wong, Carol A. Pollock, Sonia Saad
The renal consequences of maternal obesity in offspring are overwhelmed by postnatal high fat diet
www.plosone.org
www.plosone.org
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(\(dilution)Tj
3.753 0 Td
(1:200,)Tj
2.5682 0 Td
(Cell)Tj
1.8028 0 Td
(Signaling)Tj
-32.0539 -1.3039 Td
(Technology,)Tj
5.159 0 Td
(Beverly,)Tj
3.4015 0 Td
(USA\))Tj
2.4548 0 Td
(at)Tj
0.9524 0 Td
(4C)Tj
1.7518 0 Td
(overnight,)Tj
4.3087 0 Td
(followed)Tj
3.6453 0 Td
(by)Tj
1.1849 0 Td
(horseradish)Tj
4.8982 0 Td
(peroxidase)Tj
4.5184 0 Td
(anti-rabbit)Tj
-32.275 -1.2982 Td
(Envision)Tj
3.7417 0 Td
(system)Tj
2.9366 0 Td
(\(Dako)Tj
2.7383 0 Td
(Tokyo,)Tj
3.0217 0 Td
(Japan\))Tj
2.8233 0 Td
(the)Tj
1.4683 0 Td
(following)Tj
3.9798 0 Td
(day.)Tj
-19.5135 -1.2983 Td
(Alternatively,)Tj
5.6012 0 Td
(frozen)Tj
2.7666 0 Td
(sections)Tj
3.4129 0 Td
(were)Tj
2.1203 0 Td
(incubated)Tj
4.1782 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(widely)Tj
2.8233 0 Td
(used)Tj
2.058 0 Td
(markers)Tj
3.4979 0 Td
(of)Tj
1.0148 0 Td
(murine)Tj
3.1804 0 Td
(macro-)Tj
-34.3556 -1.2983 Td
(phage)Tj
2.5851 0 Td
(populations,)Tj
5.1761 0 Td
(rat)Tj
1.3266 0 Td
(anti-mouse)Tj
4.7565 0 Td
(F4/80)Tj
2.5058 0 Td
(monoclonal)Tj
5.0059 0 Td
(antibody)Tj
3.7644 0 Td
(\(dilution)Tj
3.7474 0 Td
(1:100,)Tj
2.5738 0 Td
(ABD)Tj
2.2224 0 Td
(Serotec,)Tj
-33.664 -1.3039 Td
(USA\))Tj
2.4547 0 Td
(or)Tj
1.0999 0 Td
(rat)Tj
1.3209 0 Td
(anti-mouse)Tj
4.7565 0 Td
(CD68)Tj
2.5739 0 Td
(antibody)Tj
3.7643 0 Td
(\(dilution)Tj
3.7474 0 Td
(1:100,)Tj
2.5739 0 Td
(ABD)Tj
2.2223 0 Td
(Serotec\))Tj
3.4809 0 Td
(for)Tj
1.389 0 Td
(one)Tj
1.6951 0 Td
(hour.)Tj
2.3811 0 Td
(Thereaf-)Tj
-33.4599 -1.2982 Td
(ter,)Tj
1.5363 0 Td
(they)Tj
1.9276 0 Td
(were)Tj
2.1203 0 Td
(incubated)Tj
4.1782 0 Td
(with)Tj
1.9956 0 Td
(a)Tj
0.652 0 Td
(secondary)Tj
4.2746 0 Td
(HRP)Tj
2.1656 0 Td
(labelled)Tj
3.2769 0 Td
(goat)Tj
1.9218 0 Td
(anti-rat)Tj
3.2258 0 Td
(antibody)Tj
3.7644 0 Td
(for)Tj
1.3833 0 Td
(30)Tj
1.1736 0 Td
(min)Tj
-33.596 -1.2983 Td
(\(ABD)Tj
2.5568 0 Td
(Serotec,)Tj
3.3675 0 Td
(dilution)Tj
3.4129 0 Td
(1:200\).)Tj
-8.141 -1.3039 Td
(Antigen-antibody)Tj
7.353 0 Td
(reactions)Tj
3.8551 0 Td
(were)Tj
2.1203 0 Td
(visualized)Tj
4.1442 0 Td
(with)Tj
1.9956 0 Td
(3.3diaminobenzidine)Tj
8.6966 0 Td
(tetrahydrochloride)Tj
-29.361 -1.2983 Td
(\(Dako\))Tj
3.0727 0 Td
(and)Tj
1.7291 0 Td
(counterstained.)Tj
6.4063 0 Td
(The)Tj
1.7801 0 Td
(tissue)Tj
2.4605 0 Td
(specimens)Tj
4.3653 0 Td
(were)Tj
2.1203 0 Td
(examined)Tj
4.1329 0 Td
(by)Tj
1.1792 0 Td
(light)Tj
2.0239 0 Td
(microscopy)Tj
4.8756 0 Td
(using)Tj
-34.1459 -1.2983 Td
(the)Tj
1.4683 0 Td
(Olympus)Tj
3.8948 0 Td
(photomicroscope.)Tj
7.421 0 Td
(For)Tj
1.6271 0 Td
(fibronectin,)Tj
4.8926 0 Td
(collagen)Tj
3.5149 0 Td
(IV,)Tj
1.4797 0 Td
(8-OHdg,)Tj
3.7814 0 Td
(CD68,)Tj
2.7949 0 Td
(and)Tj
1.7291 0 Td
(F4/80,)Tj
2.7326 0 Td
(six)Tj
-35.3364 -1.3039 Td
(consecutive)Tj
4.8755 0 Td
(non-overlapping)Tj
6.9278 0 Td
(fields)Tj
2.3358 0 Td
(from)Tj
2.2053 0 Td
(each)Tj
2.0296 0 Td
(section)Tj
3.0444 0 Td
(of)Tj
1.0148 0 Td
(renal)Tj
2.245 0 Td
(cortex)Tj
2.7099 0 Td
(were)Tj
2.1203 0 Td
(photographed)Tj
-29.5084 -1.2982 Td
(under)Tj
2.6191 0 Td
(high)Tj
2.007 0 Td
(magnification.)Tj
6.015 0 Td
(Stained)Tj
3.1918 0 Td
(areas)Tj
2.2451 0 Td
(were)Tj
2.1203 0 Td
(quantified)Tj
4.3086 0 Td
(using)Tj
2.3924 0 Td
(Image)Tj
2.6986 0 Td
(J)Tj
0.5499 0 Td
(software)Tj
3.5886 0 Td
(\(NIH,)Tj
2.6306 0 Td
(UK\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 524.0692 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(RT)Tj
1.5448 0 Td
(PCR)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 507.0614 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(RNA)Tj
2.262 0 Td
(was)Tj
1.6951 0 Td
(extracted)Tj
3.8891 0 Td
(and)Tj
1.7291 0 Td
(RT-PCR)Tj
3.6454 0 Td
(was)Tj
1.7007 0 Td
(performed)Tj
4.4674 0 Td
(as)Tj
1.0148 0 Td
(previously)Tj
4.354 0 Td
(described)Tj
4.0535 0 Td
([)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(].)Tj
2.075 0 Td
(PCR)Tj
2.0636 0 Td
(primers)Tj
3.3505 0 Td
(are)Tj
-36.3002 -1.3039 Td
(previously)Tj
4.3483 0 Td
(published)Tj
4.1272 0 Td
([)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(],)Tj
2.0749 0 Td
(or)Tj
1.0999 0 Td
(are)Tj
1.4456 0 Td
(available)Tj
3.668 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.4683 0 Td
(authors)Tj
3.2485 0 Td
(upon)Tj
2.3244 0 Td
(request.)Tj
3.3562 0 Td
(The)Tj
1.7801 0 Td
(results)Tj
2.812 0 Td
(are)Tj
1.4457 0 Td
(pre-)Tj
-35.4045 -1.2982 Td
(sented)Tj
2.8006 0 Td
(as)Tj
1.0148 0 Td
(fold)Tj
1.7914 0 Td
(change)Tj
3.0388 0 Td
(compared)Tj
4.2462 0 Td
(to)Tj
1.0262 0 Td
(control)Tj
3.1181 0 Td
(after)Tj
2.0352 0 Td
(normalisation)Tj
5.8337 0 Td
(to)Tj
/F11 1 Tf
1.0261 0 Td
()Tj
/F5 1 Tf
(-actin.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 453.7133 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Statistical)Tj
4.4504 0 Td
(methods)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 436.7055 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Results)Tj
3.0557 0 Td
(are)Tj
1.4513 0 Td
(expressed)Tj
4.0932 0 Td
(as)Tj
1.0148 0 Td
(mean)Tj
/F10 1 Tf
2.4491 0 Td
[()]TJ
/F5 1 Tf
0.7881 0 Td
(standard)Tj
3.719 0 Td
(error)Tj
2.2677 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(mean)Tj
2.4435 0 Td
(\(SEM\).)Tj
3.067 0 Td
(Data)Tj
2.1203 0 Td
(were)Tj
2.126 0 Td
(analysed)Tj
3.6453 0 Td
(using)Tj
-34.7241 -1.2982 Td
(analysis)Tj
3.3278 0 Td
(of)Tj
1.0148 0 Td
(variance)Tj
3.5716 0 Td
(\(ANOVA\),)Tj
4.6715 0 Td
(and)Tj
1.7348 0 Td
(post-hoc)Tj
3.7077 0 Td
(Bonferroni)Tj
4.6318 0 Td
(tests)Tj
1.9672 0 Td
(to)Tj
1.0205 0 Td
(make)Tj
2.3981 0 Td
(between-group)Tj
6.2645 0 Td
(com-)Tj
-34.3103 -1.3039 Td
(parisons.)Tj
3.8097 0 Td
(To)Tj
1.3379 0 Td
(compare)Tj
3.7191 0 Td
(plasma)Tj
3.033 0 Td
(glucose)Tj
3.1691 0 Td
(levels)Tj
2.3754 0 Td
(during)Tj
2.9311 0 Td
(the)Tj
1.4683 0 Td
(IPGTT,)Tj
3.2825 0 Td
(a)Tj
0.652 0 Td
(two-way)Tj
3.6339 0 Td
(ANOVA)Tj
3.7644 0 Td
(was)Tj
1.7008 0 Td
(per-)Tj
-34.8772 -1.2983 Td
(formed)Tj
3.1577 0 Td
(and)Tj
1.7291 0 Td
(between-group)Tj
6.2646 0 Td
(differences)Tj
4.5694 0 Td
(were)Tj
2.1203 0 Td
(determined)Tj
4.8529 0 Td
(using)Tj
2.3867 0 Td
(Tukey's)Tj
3.3109 0 Td
(post)Tj
1.9049 0 Td
(hoc)Tj
1.6781 0 Td
(test.)Tj
1.8255 0 Td
(Analyses)Tj
-33.8001 -1.2983 Td
(were)Tj
2.1203 0 Td
(carried)Tj
3.0387 0 Td
(out)Tj
1.5533 0 Td
(using)Tj
2.3925 0 Td
(GraphPad)Tj
4.3029 0 Td
(Prism)Tj
2.5966 0 Td
(6.0)Tj
1.4003 0 Td
(\(GraphPad)Tj
4.6431 0 Td
(Software,)Tj
3.9288 0 Td
(San)Tj
1.6781 0 Td
(Diego,)Tj
2.8459 0 Td
(USA\))Tj
2.4548 0 Td
(and)Tj
1.7292 0 Td
(P)Tj
-34.6845 -1.3039 Td
(value)Tj
/F12 1 Tf
0 Tc
2.3073 0 Td
(<)Tj
/F5 1 Tf
-0.004 Tc
0.9865 0 Td
(0.05)Tj
1.8765 0 Td
(was)Tj
1.6951 0 Td
(considered)Tj
4.6035 0 Td
(statistically)Tj
4.6091 0 Td
(significant.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 344.3527 cm
BT
/F2 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Results)Tj
/F0 1 Tf
0 -1.5023 Td
(Maternal)Tj
4.063 0 Td
(obesity)Tj
3.3496 0 Td
(increases)Tj
4.4504 0 Td
(adiposity)Tj
4.1103 0 Td
(in)Tj
0.9827 0 Td
(the)Tj
1.5969 0 Td
(presence)Tj
4.285 0 Td
(of)Tj
1.0441 0 Td
(obesity)Tj
3.3497 0 Td
(and)Tj
1.8661 0 Td
(STZ)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 309.3732 cm
BT
/F5 1 Tf
1 TL
-0.004 Tc
0 0 Td
(As)Tj
1.2642 0 Td
(expected,)Tj
3.9458 0 Td
(animals)Tj
3.3335 0 Td
(fed)Tj
1.457 0 Td
(HFD)Tj
2.2507 0 Td
(from)Tj
2.2054 0 Td
(weaning)Tj
3.5943 0 Td
(until)Tj
2.1089 0 Td
(Week)Tj
2.5285 0 Td
(32)Tj
1.1679 0 Td
(had)Tj
1.7121 0 Td
(increased)Tj
3.9968 0 Td
(body)Tj
2.2167 0 Td
(weight)Tj
2.8856 0 Td
(com-)Tj
-34.6674 -1.3039 Td
(pared)Tj
2.4944 0 Td
(to)Tj
1.0262 0 Td
(control)Tj
3.118 0 Td
(\(P)Tj
/F12 1 Tf
0 Tc
1.1226 0 Td
(<)Tj
/F5 1 Tf
-0.004 Tc
0.9864 0 Td
(0.0001)Tj
2.829 0 Td
(for)Tj
1.3833 0 Td
(all)Tj
1.1452 0 Td
(groups)Tj
2.9706 0 Td
(vs.)Tj
1.2586 0 Td
(CC;)Tj
0.83 0.64 0.02 0 k
1.7745 0 Td
(Fig)Tj
1.4797 0 Td
(1A)Tj
0 g
(\).)Tj
1.9445 0 Td
(There)Tj
2.5795 0 Td
(was)Tj
1.6951 0 Td
(no)Tj
1.2756 0 Td
(difference)Tj
4.2066 0 Td
(in)Tj
1.0375 0 Td
(body)Tj
-34.3273 -1.2982 Td
(weight)Tj
2.8856 0 Td
(of)Tj
1.0148 0 Td
(offspring)Tj
3.8324 0 Td
(of)Tj
1.0148 0 Td
(obese)Tj
2.4435 0 Td
(mothers)Tj
3.5262 0 Td
(weaned)Tj
3.2599 0 Td
(to)Tj
1.0261 0 Td
(a)Tj
0.6576 0 Td
(normal)Tj
3.1465 0 Td
(chow)Tj
2.3584 0 Td
(diet)Tj
1.7404 0 Td
(compared)Tj
4.2406 0 Td
(to)Tj
1.0262 0 Td
(control.)Tj
-32.173 -1.2983 Td
(Maternal)Tj
3.8664 0 Td
(obesity)Tj
3.0387 0 Td
(was)Tj
1.7008 0 Td
(associated)Tj
4.2576 0 Td
(with)Tj
1.9956 0 Td
(increased)Tj
4.0025 0 Td
(body)Tj
2.211 0 Td
(weight)Tj
2.8913 0 Td
(in)Tj
1.0318 0 Td
(offspring)Tj
3.8324 0 Td
(fed)Tj
1.4627 0 Td
(HFD)Tj
2.2507 0 Td
(and)Tj
1.7291 0 Td
(given)Tj
2.3811 0 Td
(a)Tj
-36.6517 -1.3039 Td
(further)Tj
3.0273 0 Td
(diabetes-related)Tj
6.5367 0 Td
(insult)Tj
2.4661 0 Td
(with)Tj
2.0013 0 Td
(STZ)Tj
1.9048 0 Td
(\(CH-STZ)Tj
4.0309 0 Td
(vs.)Tj
1.2585 0 Td
(HH-STZ,)Tj
4.0195 0 Td
(P)Tj
/F12 1 Tf
0 Tc
0.7824 0 Td
(<)Tj
/F5 1 Tf
-0.004 Tc
0.9921 0 Td
(0.01\).)Tj
-25.8234 -1.2983 Td
(Visceral)Tj
3.4412 0 Td
(adiposity,)Tj
4.0819 0 Td
(as)Tj
1.0148 0 Td
(measured)Tj
4.1102 0 Td
(by)Tj
1.1792 0 Td
(retroperitoneal)Tj
6.2305 0 Td
(and)Tj
1.7291 0 Td
(epididymal)Tj
4.7055 0 Td
(fat)Tj
1.2416 0 Td
(mass,)Tj
2.4207 0 Td
(was)Tj
1.6951 0 Td
(increased)Tj
-33.046 -1.2982 Td
(in)Tj
1.0318 0 Td
(all)Tj
1.1508 0 Td
(groups)Tj
2.965 0 Td
(fed)Tj
1.4627 0 Td
(HFD)Tj
2.2507 0 Td
(\(P)Tj
/F12 1 Tf
0 Tc
1.1168 0 Td
(<)Tj
/F5 1 Tf
-0.004 Tc
0.9922 0 Td
(0.0001;)Tj
0.83 0.64 0.02 0 k
3.05 0 Td
(Fig)Tj
1.4797 0 Td
(1B)Tj
1.2756 0 Td
(and)Tj
1.7291 0 Td
(1C)Tj
0 g
(\).)Tj
1.9275 0 Td
(Similar)Tj
3.1011 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(observation)Tj
4.8982 0 Td
(for)Tj
1.389 0 Td
(body)Tj
2.211 0 Td
(weight,)Tj
-34.5257 -1.304 Td
(offspring)Tj
3.8267 0 Td
(of)Tj
1.0091 0 Td
(obese)Tj
2.4378 0 Td
(mothers)Tj
3.5206 0 Td
(fed)Tj
1.457 0 Td
(HFD)Tj
2.245 0 Td
(and)Tj
1.7235 0 Td
(given)Tj
2.3754 0 Td
(one)Tj
1.6951 0 Td
(dose)Tj
2.0296 0 Td
(of)Tj
1.0091 0 Td
(STZ)Tj
1.9049 0 Td
(had)Tj
1.7008 0 Td
(significantly)Tj
5.0853 0 Td
(increased)Tj
3.9968 0 Td
(adi-)Tj
-36.0167 -1.2982 Td
(posity)Tj
2.6248 0 Td
(as)Tj
1.0148 0 Td
(measured)Tj
4.1102 0 Td
(by)Tj
1.1792 0 Td
(both)Tj
2.058 0 Td
(fat)Tj
1.2415 0 Td
(depots)Tj
2.8573 0 Td
(compared)Tj
4.2406 0 Td
(to)Tj
1.0262 0 Td
(similar)Tj
2.9877 0 Td
(offspring)Tj
3.8324 0 Td
(of)Tj
1.0148 0 Td
(lean)Tj
1.8708 0 Td
(mothers)Tj
3.532 0 Td
(\(CH-STZ)Tj
-33.5903 -1.2983 Td
(vs.)Tj
1.2585 0 Td
(HH-STZ,)Tj
4.0195 0 Td
(P)Tj
/F12 1 Tf
0 Tc
0.7767 0 Td
(<)Tj
/F5 1 Tf
-0.004 Tc
0.9921 0 Td
(0.05\).)Tj
-5.8506 -1.2983 Td
(All)Tj
1.4003 0 Td
(groups)Tj
2.9707 0 Td
(fed)Tj
1.457 0 Td
(HFD)Tj
2.2507 0 Td
(demonstrated)Tj
5.7599 0 Td
(hepatomegaly)Tj
5.7713 0 Td
(except)Tj
2.7723 0 Td
(for)Tj
1.389 0 Td
(the)Tj
1.4683 0 Td
(CH-STZ.)Tj
3.9118 0 Td
(Though)Tj
3.3902 0 Td
(there)Tj
2.262 0 Td
(was)Tj
-35.9997 -1.3039 Td
(no)Tj
1.2755 0 Td
(effect)Tj
2.3641 0 Td
(of)Tj
1.0148 0 Td
(maternal)Tj
3.7927 0 Td
(obesity)Tj
3.0388 0 Td
(on)Tj
1.2755 0 Td
(liver)Tj
1.9843 0 Td
(mass)Tj
2.194 0 Td
(in)Tj
1.0375 0 Td
(offspring)Tj
3.8324 0 Td
(fed)Tj
1.4626 0 Td
(HFD,)Tj
2.4718 0 Td
(offspring)Tj
3.8381 0 Td
(fed)Tj
1.457 0 Td
(HFD)Tj
2.2507 0 Td
(and)Tj
1.7291 0 Td
(given)Tj
-35.0189 -1.2982 Td
(STZ)Tj
1.9048 0 Td
(had)Tj
1.7121 0 Td
(increased)Tj
3.9969 0 Td
(liver)Tj
1.9842 0 Td
(size)Tj
1.6838 0 Td
(\(CH-STZ)Tj
4.0308 0 Td
(vs.)Tj
1.2586 0 Td
(HH-STZ,)Tj
4.0195 0 Td
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