TY  - JOUR
AB  - The CRISPR-Cas9 and related systems offer a unique genome-editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with the CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with the CRISPR-Cas9 system. We demonstrated its efficient protein release by respectively assessing the targeted knockout of the eGFP gene in human HEK293/GFP cells and the TNFAIP3 gene in TNF?-induced HEK293 cells. We further validated our results at a single-cell resolution using an <i>in vivo</i> eGFP reporter system in zebrafish (77% knockout). These findings indicate that light-triggered liposomes may have new options for precise control of CRISPR-Cas9 release and editing.
AU  - Aksoy, YA
AU  - Yang, B
AU  - Chen, W
AU  - Hung, T
AU  - Kuchel, RP
AU  - Zammit, NW
AU  - Grey, ST
AU  - Goldys, EM
AU  - Deng, W
DA  - 2020/11/11
DO  - 10.1021/acsami.0c16380
EP  - 52444
JO  - ACS applied materials & interfaces
PB  - AMER CHEMICAL SOC
PY  - 2020/11/11
SP  - 52433
TI  - Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System.
VL  - 12
Y1  - 2020/11/11
Y2  - 2026/05/21
ER  -