TY  - CHAP
AB  - Because they are firmly anchored to a noble metal substrate, tethered bilayer lipid membranes (tBLMs) are considerably more robust than supported lipid bilayers such as black lipid membranes (BLMs) (Cranfield et al. Biophys J 106:182-189, 2014). The challenge to rapidly create asymmetrical tBLMs that include a lipopolysaccharide outer leaflet for bacterial model membrane research can be overcome by the use of a Langmuir-Schaefer deposition protocol. Here, we describe the procedures required to assemble and test asymmetric lipopolysaccharide (LPS) tethered lipid bilayers.
AU  - Cranfield, CG
AU  - Le Brun, AP
AU  - Garcia, A
AU  - Cornell, BA
AU  - Holt, SA
DA  - 2022
DO  - 10.1007/978-1-0716-1843-1_3
EP  - 30
JO  - Methods in Molecular Biology
PB  - Springer US
PY  - 2022
SP  - 21
TI  - Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely Tethered Lipid Bilayer.
VL  - 2402
Y1  - 2022
Y2  - 2026/08/03
ER  -