TY  - JOUR
AB  - We report an aggregation-induced emission fluorogen (AIEgen)-based turn-on fluorescent aptasensor able to detect the ultrasmall concentration of intracellular IFN-?. The aptasensor consists of an IFN-? aptamer labeled with a fluorogen with a typical aggregation-induced emission (AIE) characteristic, which shows strong red emission only in the presence of IFN-?. The aptasensor is able to effectively monitor intracellular IFN-? secretion with the lowest detection limit of 2 pg mL-1, and it is capable of localizing IFN-? in live cells during secretion, with excellent cellular permeability and biocompatibility as well as low cytotoxicity. This probe is able to localize the intracellular IFN-? at a low concentration <10 pg mL-1, and it is successfully used for real-time bioimaging. This simple and highly sensitive sensor may enable the exploration of cytokine pathways and their dynamic secretion process in the cellular environment. It provides a universal sensing platform for monitoring a spectrum of molecules secreted by cells.
AU  - Ma, K
AU  - Zhang, F
AU  - Sayyadi, N
AU  - Chen, W
AU  - Anwer, AG
AU  - Care, A
AU  - Xu, B
AU  - Tian, W
AU  - Goldys, EM
AU  - Liu, G
DA  - 2018/02/23
DO  - 10.1021/acssensors.7b00720
EP  - 326
JO  - ACS Sens
PB  - AMER CHEMICAL SOC
PY  - 2018/02/23
SP  - 320
TI  - "Turn-on" Fluorescent Aptasensor Based on AIEgen Labeling for the Localization of IFN-? in Live Cells.
VL  - 3
Y1  - 2018/02/23
Y2  - 2026/05/10
ER  -