http://hdl.handle.net/10453/148698 2026-06-06T02:42:09Z http://hdl.handle.net/10453/195221 Title: Fermented rice bran extract delays skin aging by increasing the synthesis of collagen and elastin. Authors: Yan, X; Yang, M; Cai, X; Shen, Y; Jiang, R; Huang, R; Shi, H-K; Cheng, G; Wang, Y; Tan, Q; Cheng, YY; Xue, N Abstract: BACKGROUND: Rice bran is the outer layer of rice grains (Oryza sativa). Due to its rich bioactive components, it has long been used in cosmetics. However, the mechanism by which it delays skin aging remains unclear. METHODS: In this study, volatile polar solvents combined with microbial fermentation were utilized to enhance the yield and bioavailability of functional components in rice bran extract (RBE). The crude RBE was fermented with Aspergillus oryzae for 14 days to promote enzymatic decomposition into smaller and more bioavailable molecules. The components in the fermented RBE were qualitatively analyzed by UPLC-QTOF-MS/MS. The expression of collagen in two-dimensional and three-dimensional cell cultures was evaluated by qPCR technology. The expressions of collagen and elastin and the changes in water content and elastic modulus in the skin of mice were evaluated by histopathology, immunofluorescence staining, and transepidermal water loss (TEWL). RESULTS: Through UPLC-QTOF-MS/MS analysis, eight key compounds, including azelaic acid, ferulic acid, γ-tocotrienols, and squalene, were identified in RBE, mainly lipids and polyphenols. The treatment of RBE significantly upregulated the expression of type I collagen in MSF cells and the expression of type III collagen in MSF 3D cell spheres (by approximately 12 times). The results of tissue staining showed that the content of collagen in the skin after RBE treatment increased by 10% compared with the control group. The results of immunofluorescence staining confirmed that RBE could increase the content of elastin in the skin. The TEWL results showed that the skin moisture content and elastic coefficient of mice treated with RBE increased by more than 10% compared with those of the untreated group. CONCLUSION: Both in vitro and in vivo studies have shown that RBE can significantly improve the synthesis of collagen and elastin in the skin, reduce water loss in mouse skin, increase collagen deposition in the skin, and ultimately improve skin elasticity and overall quality. This green, solvent-efficient, and fermentation-enhanced approach offers a sustainable strategy for utilizing rice bran as a high-value cosmetic ingredient with strong potential for skincare applications. 2025-01-01T00:00:00Z http://hdl.handle.net/10453/195183 Title: Advances in nanopore direct RNA sequencing and its impact on biological research. Authors: Sun, K; Li, J; Chen, C; Zhou, X; Ma, G; Mao, L; Tang, Q; Ma, B; Li, D; Chen, Z; Cen, C; Shentu, X; Ye, Z; Yu, X Abstract: Nanopore direct RNA sequencing (DRS) is a transformative technology that enables full-length, single-molecule sequencing of native RNA, capturing transcript isoforms and preserving epitranscriptomic modifications without cDNA conversion. This review outlines key advances in DRS, including optimized protocols for mRNA, rRNA, tRNA, circRNA, and viral RNA, as well as analytical tools for isoform quantification, poly(A) tail measurement, fusion transcript identification, and base modification profiling. We highlight how DRS has redefined transcriptomic studies across diverse systems-from uncovering novel transcripts and alternative splicing events in cancer, plants, and parasites to enabling the direct detection of m6A, m5C, pseudouridine, and RNA editing events. Emerging applications such as co-transcriptional splicing analysis, lncRNA and circRNA discovery, and real-time RNA structural mapping are also discussed. Beyond basic research, DRS offers powerful capabilities in mRNA vaccine quality control and RNA-based data storage. Despite current limitations in sequencing accuracy, input requirements, and cost, ongoing improvements in nanopore chemistry, basecalling algorithms, and machine learning integration are rapidly expanding DRS utility. As it matures, DRS is poised to become a core platform for high-resolution transcriptome profiling, RNA regulatory analysis, and integrative multi-omics applications, offering novel insights into gene expression, regulation, and evolution. 2025-12-01T00:00:00Z http://hdl.handle.net/10453/195126 Title: DENOSUMAB: A NEW ERA IN OSTEOPOROSIS MANAGEMENT IN VIETNAM Authors: Van Tuan, N; Pham Thuc Lan, H Abstract: Osteoporosis is a skeletal disorder characterized by compromised bone strength and increased fracture risk. In Vietnam, the disease affects 30 in postmenopausal women and 10 in men over 50, highlighting a growing public health challenge as the population ages. Bisphosphonates remain the standard firstline treatment. Recent advances in bone biology highlight the critical role of the RANK/RANKL/OPG pathway in bone remodeling. Osteoporosis arises when the RANKL/OPG ratio becomes imbalanced. Denosumab, a monoclonal antibody that targets RANKL, replicates OPG s ability to suppress osteoclast activity, increasing bone mineral density and reducing fracture risk by 20 68 across various skeletal sites. Unlike bisphosphonates, Denosumab is reversible, metabolized by the reticuloendothelial system, and appropriate for patients with advanced kidney disease, though it necessitates monitoring for hypocalcemia. This advancement offers clinicians a valuable tool for managing osteoporosis in Vietnam. http://hdl.handle.net/10453/195125 Title: BIẾN NGHIÊN CỨU TRỞ NÊN CÓ GIÁ TRỊ: ÁP DỤNG PICOT VÀ POEM CHO NGHIÊN CỨU LÂM SÀNG Authors: Văn Tuấn, N Abstract: Ph t bi u m t c u h i nghi n c u r r ng v c c u tr c l b c u quan tr ng cho s th nh c ng c a nghi n c u y h c. Tuy nhi n, nhi u b c s l m s ng g p kh kh n v i b c kh i u n y, d n n nghi n c u thi u t p trung v t c kh n ng t c ng ng k n th c h nh. Trong b i, t i gi i thi u khung PICOT c th bi n nh ng t m m h th nh c c c u h i ch nh x c, c th tr l i, ph h p v i ph ng ph p nghi n c u th c ti n. Tuy nhi n, m t c u h i c c u tr c t t v n ch a n u n kh ng d n n k t qu ngh a cho b nh nh n. Nguy n t c POEM cung c p m t b l c nh gi c c c u h i nghi n c u, b o m r ng c c nghi n c u t p trung v o k t qu li n quan n s c kh e c a b nh nh n, thay v ch t p trung v o c c ch s sinh ho . PICOT v POEM l hai nguy n t c c t l i c a y h c th c ch ng, gi p c c b c s l m s ng u ti n nghi n c u s thay i t ch c c th c h nh l m s ng v tr c ti p mang l i l i ch cho b nh nh n, b o m r ng th i gian v ngu n l c c u t v o nghi n c u t o ra b ng ch ng th c s c th p d ng t i gi ng b nh. Formulating a clear and structured research question is a critical first step for the success of a medical study. However, many clinicians struggle with this initial phase, leading to unfocused research with little potential for significant impact on practice. In this paper, I introduce the PICOT framework, which can transform vague curiosities into precise, answerable questions suitable for practical research methodologies.However, a well-structured question is insufficient if it does not lead to outcomes that are meaningful to patients. The POEM (Patient-Oriented Evidence that Matters) principle provides a filter for evaluating research questions, ensuring that studies focus on outcomes relevant to patient health, rather than solely on biochemical markers. PICOT and POEM are two core principles of evidence-based medicine that help clinicians prioritize research that will positively change clinical practice and directly benefit patients. This ensures that time and resources are invested in research that generates ev