http://hdl.handle.net/10453/148698 2026-05-17T02:27:46Z http://hdl.handle.net/10453/195004 Title: A Computational Approach for Biomimetic Design of Liver‐On‐A‐Chip Authors: Yang, Z; Yin, Q; Liu, Q; Xu, Z; Zhang, Y; Vega‐Sánchez, C; Liu, X; Vigolo, D; Li, JJ; Yong, K Abstract: In this study, we present a biomimetic liver‐on‐a‐chip (LOC) device inspired by the structural organisation of the hepatic acinus and developed through COMSOL Multiphysics simulation. The design incorporates antiparallel perfusion channels and microchannel barriers to mimic nutrient delivery and bile drainage in the liver, while maintaining physiologically relevant low shear stress conditions suitable for hepatocyte culture. A truncated LOC model was first used to explore pressure, shear stress, and concentration distributions, revealing that media perfusion minimally perturbs the cell region while bile flow significantly impacts nutrient diffusion. Full‐scale simulations were then performed, demonstrating key discrepancies with the truncated model due to length‐dependent diffusion effects, emphasising the importance of simulating full geometries in transport‐limited systems. The model was validated experimentally with dye‐based flow studies and HepG2 cell culture under dynamic perfusion. Functional validation confirmed that the LOC supports directional solute gradients and cell growth under controlled shear. This integrated in silico–in vitro approach provides a robust framework for the design of organ‐on‐a‐chip systems, reducing development cycles and improving physiological relevance for drug testing and liver disease modelling. http://hdl.handle.net/10453/194980 Title: Case study and literature review: the role of selective venous sampling in the management of challenging primary hyperparathyroidism Authors: Hashmi, A 2025-02-01T00:00:00Z http://hdl.handle.net/10453/194973 Title: 358 INCIDENCE OF AND RISK FACTORS FOR RADIOGRAPHIC KNEE OSTEOARTHRITIS: THE VIETNAM OSTEOPOROSIS STUDY Authors: Hoang, KD; Truong, TA; Luu, NL; Nguyen, GH; Hunter, DJ; Ho-Pham, LT; Nguyen, TV 2025-04-01T00:00:00Z http://hdl.handle.net/10453/194945 Title: Numerical simulation analysis of flow field and fabrication of cells-osteochondral scaffold constructs in a spinner flask bioreactor. Authors: Hu, X; Chen, H; Ma, H; Zhu, J; Cheng, YY; Xu, H; Song, K Abstract: With the vigorous development of bone/cartilage tissue engineering research, the construction system for in vitro preparation of tissue engineered osteochondral repair substitutes is undergoing a transformation from static culture mode to 3D dynamic culture mode. However, for dynamic culture mode, many problems such as the selection of cultivation environment and the optimization of condition parameters need to be solved. In this study, computational fluid dynamics (CFD) was used to simulate and predict the stress conditions of adipose derived stem cells-chitosan7/gelatin3/Nano-hydroxyapatite (ADSCs-Cs7/Gel3/nHAP) structures and ADSCs-bone-derived scaffold structures, as well as the flow field distribution in spinner flask (SF) at different rotational speeds. Finally, the appropriate operating conditions of SF were optimized. The simulation results showed that SF generated two fluid cycles bounded by the bottom edge of the stirring paddle in the entire fluid flow region, with a fluid circulation region exhibiting a relatively static flow field distribution (compared with the first two cycles) directly below the stirring axis. There was a moderate dynamic pressure and speed under the stirring paddle, making this area the most suitable for fixing the cell-scaffold constructs. Under different rotational speed conditions, the dynamic pressure and fluid shear force of the constructs in SF were positively correlated with the speed. Overall, considering all factors, 50 rpm and 70 rpm were determined as the preferred rotational speed conditions for the ADSCs-Cs7/Gel3/nHAP constructs and ADSCs-derived-bone scaffold constructs in SF, respectively. Subsequently, the cell-scaffold complex cultured under SF was implanted at the site of osteochondral defect in New Zealand rabbits, and it was found that new tissues were formed after 4 weeks of culture. These results indicate that SF cultured scaffolds are suitable for repairing rabbit osteochondral defects. 2025-10-01T00:00:00Z