http://hdl.handle.net/10453/35351 2026-10-15T05:39:16Z http://hdl.handle.net/10453/196160 Title: Altered Abundance of Barrier-Related Proteins in Brain Microvascular Endothelial Cells of the GL261 Mouse Model of Glioblastoma. Authors: Koehn, LM; Cao, D; Steele, JR; Rigopoulos, A; Burvenich, IJ; Chung-Lee, H; Tanuwidjaya, E; Schittenhelm, RB; Scott, AM; Gan, HK; Nicolazzo, JA Abstract: Glioblastoma multiform (GBM) is a brain cancer that has limited treatment options and a high fatality rate, due in part to limited access of chemotherapeutics to the tumor resulting from the restrictive nature of the blood-brain barrier (BBB). The present study characterized the proteome of endothelial cells forming the BBB in a mouse model of GBM, as a way to identify putative transporters that could be exploited to enhance drug delivery in GBM. Female 6-8 week old C57BL/6 mice were intracranially injected with glioma 261 (GL261) cells or underwent a sham injection. After 28-29 days, brain endothelial cells (CD31+/CD45-) from GL261 (GBM-EC) and sham-injected (control-EC) mice were isolated using magnetic-activated cell sorting, and the proteome of cells was compared by untargeted liquid chromatography dual mass spectrometry. GBM-EC had significantly lower abundance of tight junction proteins (e.g., tight junction protein 1, 0.4-fold) and drug-metabolizing enzymes (e.g., glutathione-S-transferase A4, 0.4-fold) compared to control-EC, alongside an up- and down-regulation of drug transporters (e.g., long-chain fatty acid transport protein 4, 5-fold; adenosine triphosphate binding cassette transporter subfamily B member 1A, 0.3-fold). A large, 7-fold up-regulation of the endothelial cell surface receptor melanoma cell adhesion molecule (MCAM) and scavenger receptor class B member 1 (SCARB1) were identified in GBM-EC compared to control-EC. Immunohistochemistry confirmed cerebral endothelial localization of MCAM and SCARB1 in GBM, in addition to nonvascular patterning within the GBM, suggesting these receptors may be targets that could be exploited for drug delivery. The present study identified changes to BBB markers of paracellular permeability, as well as active and receptor-mediated transcellular transport that could present novel avenues to consider to enhance the permeability and GBM access of current and future therapeutics. 2025-11-03T00:00:00Z http://hdl.handle.net/10453/195024 Title: Clostridioides difficile in feral horse populations in Australia Authors: Hain-Saunders, NMR; Knight, DR; Harvey, A; Bruce, M; Hampson, BA; Riley, TV Editors: Vives, M Abstract: i Clostridioides difficile /i is a known cause of diarrhea and colitis in human and non-human animals. While i C. difficile /i is regularly isolated from domesticated horses, little is known about its prevalence in wild or feral populations. In Australia, the horse population encompasses a mix of both domesticated and feral animals, with the feral population of 400,000 estimated to be the largest in the world. This study investigated the presence and characteristics of i C. difficile /i in Australian feral horses and evaluated their potential as a source or reservoir of i C. difficile /i in the wider community. Fecal samples ( i n /i 380) were collected from free-roaming feral horses from five Australian jurisdictions and cultured for i C. difficile /i . Isolates were characterized by PCR ribotyping and toxin profiling. Antimicrobial susceptibility testing was performed for fidaxomicin, vancomycin, metronidazole, rifaximin, clindamycin, erythromycin, amoxicillin-clavulanate, moxifloxacin, meropenem, and tetracycline. i C. difficile /i was isolated from 45 of the 380 samples (11.8 )-one-third of that seen in recent studies on Australian domesticated horses but consistent with wild animal species worldwide. Forty ribotypes (RTs) were identified, 28 of which (70 ) were novel other RTs had been previously reported in humans, livestock, and soils. Eighteen toxigenic i C. difficile /i strains were isolated, of which eight contain binary toxin genes. Strains were largely susceptible to the antimicrobial agents tested. This investigation provides preliminary information on i C. difficile /i in feral horses in Australia and allows a comparison with their domestic counterparts. The findings support the hypothesis that all horse feces represent a potential source of i C. difficile /i in the community. IMPORTANCE i Clostridioides difficile /i poses an ongoing threat to healthcare in the community, with increasing evidence of transmission outside the hospital setting. In keepin 2025-05-21T00:00:00Z http://hdl.handle.net/10453/194378 Title: CHALLENGES AND STRATEGIES FOR INCORPORATING INDIGENOUS LAWS AND HISTORIES ACROSS LEGAL EDUCATION CURRICULUM Authors: Gainsford, A; Gerard, A; Colvin, E Abstract: Looking at institutional policy and professional standards, this chapter highlights ways of developing greater cultural safety within law schools, with a view to raising the cultural safety of the legal system more broadly. The first part of this chapter defines cultural safety and examines its significance to the process of embedding Indigenous perspectives in law schools. The authors then closely examine an institutional strategy that can support law schools to decolonise their curricula. The chapter also discusses the role of standards and requirements for admission to practice in influencing legal curricula. Finally, industry standards for health professionals are considered with a view to capturing lessons for developing professional admissions standards in law. 2025-01-01T00:00:00Z http://hdl.handle.net/10453/193880 Title: Do Pre Service Teachers Build Capacity for Inclusive Classroom Teaching During Their Teacher Education Program Authors: Auhl, G; Bain, A 2025-01-01T00:00:00Z