http://hdl.handle.net/10453/37630 2026-04-08T10:30:56Z http://hdl.handle.net/10453/193696 Title: Influence of arbuscular mycorrhizal fungi on water relations in C3/C4 grasses under extreme heat and water stress Authors: Jones, SL; van der Merwe, M; Leigh, A; Robinson, SA; Gluskie, CT; French, K 2026-01-01T00:00:00Z http://hdl.handle.net/10453/193690 Title: Unveiling the BRAF fusion structure variations through DNA and RNA sequencing. Authors: Yang, Q; Wang, B; Meng, X; Ma, T; Qian, Z; Cai, X; Sun, X; Xu, Y; Luo, Q; Yang, T; Tao, H; Qian, Y; Shao, J; Ye, Z Abstract: BACKGROUND: The detection of BRAF fusions by using next-generation sequencing (NGS) is essential for comprehensive analysis. METHODS: Data BRAF positive rearrangements from Chinese cancer patients were analyzed. DNA NGS was performed on FFPE samples, and RNA NGS was used to confirm fusion transcripts. RESULTS: BRAF fusions were identified in various cancers, predominantly glioma (87.8%). DNA NGS detected 371 BRAF fusion-positive samples, with 338 retaining the serine/threonine receptor tyrosine kinase domain (RTKD), divided into four groups: common (n = 254), rare (n = 66), intergenic (n = 7), and exonic (n = 11) fusions. Common fusions, mainly KIAA1549-BRAF, comprised the majority, with variations at introns 8, 9, and 10. Rare fusions and intergenic/exonic breakpoints displayed diverse structural patterns. RNA NGS verified transcriptional consistency in most samples from common fusions. However, various outcomes at the RNA level were found in other groups, involving mechanisms like alternative splicing, antisense rearrangement, and frameshift rearrangement. Additionally, 33 fusions lacked the RTKD, demonstrating significant structural diversity. Furthermore, 22 novel fusions were identified, which were distributed in tongue cancer, liver cancer, lung cancer, melanoma, brain cancer, and colon cancer. CONCLUSIONS: Comprehensive molecular profiling and RNA sequencing are essential for accurate fusion detection, improving the design of NGS panels and aiding in the targeted therapy of BRAF fusion-positive cancers. 2025-06-01T00:00:00Z http://hdl.handle.net/10453/193685 Title: Handling the heat: Warming does not reduce alpine plant survival or reproduction under high precipitation conditions Authors: Xirocostas, ZA; Moles, AT; Brown, FM; Brown, ZA; Chiarenza, GM; Earle, R; Leigh, A; Mu, X; Sumner, EE; Venn, SE; Williamson, VG; Zeng, K; Osmolovsky, I 2026-07-01T00:00:00Z http://hdl.handle.net/10453/193632 Title: Who gets asthma, and why? Authors: Evans, DJ; Sly, PD; Foster, P; Donovan, C 2025-11-17T00:00:00Z