Isolation and characterisation of cathepsin L1 protease from Fasciola hepatica excretory-secretory products for serodiagnosis of human fasciolosis

O'Neill, S; Mulcahy, G; Dalton, JP

Isolation and characterisation of cathepsin L1 protease from Fasciola hepatica excretory-secretory products for serodiagnosis of human fasciolosis

O'Neill, S Mulcahy, G Dalton, JP

Permalink

Publisher:: Humana Press Inc.
Publication Type:: Chapter
Citation:: Methods in Biotechnology: Food Borne Pathogens, 2006, 1, pp. 191 - 202
Issue Date:: 2006-01

Closed Access

	Filename	Description	Size
	2005002874OK.pdf		277.01 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	O'Neill, S	en_US
dc.contributor.author	Mulcahy, G	en_US
dc.contributor.author	Dalton, JP	en_US
dc.contributor.editor	Adley, CC	en_US
dc.date.issued	2006-01	en_US
dc.identifier.citation	Methods in Biotechnology: Food Borne Pathogens, 2006, 1, pp. 191 - 202	en_US
dc.identifier.isbn	1-59259-990-7	en_US
dc.identifier.uri	http://hdl.handle.net/10453/11658
dc.description.abstract	The major antigens secreted by the parasite Fasciola hepatica are cathepsin-L cysteine proteases. The enzymes can be isolated from the parasite excretory-secretory products in sufficient quantities for use as an antigen for the serodiagnosis of humna fasciolosis. The methods illustrated in this chapter willexplain the isolation of cysteine proteases from E hepatica excretory-secretory products by gel filtration and anion exchange chromatography, and their subsequent characterisation and employment in an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-fasciola antibodies int he serum of infected humans	en_US
dc.publisher	Humana Press Inc.	en_US
dc.relation.ispartof	Methods in Biotechnology: Food Borne Pathogens	en_US
dc.title	Isolation and characterisation of cathepsin L1 protease from Fasciola hepatica excretory-secretory products for serodiagnosis of human fasciolosis	en_US
dc.type	Chapter
utslib.location	New Jersey, USA	en_US
utslib.for	060107 Enzymes	en_US
utslib.for	110803 Medical Parasitology	en_US
utslib.citation.edition	1	en_US
dc.location.activity	Perth, Aust
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.consider-herdc	true	en_US
pubs.edition	1	en_US
pubs.place-of-publication	New Jersey, USA	en_US

Abstract:

The major antigens secreted by the parasite Fasciola hepatica are cathepsin-L cysteine proteases. The enzymes can be isolated from the parasite excretory-secretory products in sufficient quantities for use as an antigen for the serodiagnosis of humna fasciolosis. The methods illustrated in this chapter willexplain the isolation of cysteine proteases from E hepatica excretory-secretory products by gel filtration and anion exchange chromatography, and their subsequent characterisation and employment in an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-fasciola antibodies int he serum of infected humans

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/11658