Isolation and characterisation of cathepsin L1 protease from Fasciola hepatica excretory-secretory products for serodiagnosis of human fasciolosis

Publisher:
Humana Press Inc.
Publication Type:
Chapter
Citation:
Methods in Biotechnology: Food Borne Pathogens, 2006, 1, pp. 191 - 202
Issue Date:
2006-01
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The major antigens secreted by the parasite Fasciola hepatica are cathepsin-L cysteine proteases. The enzymes can be isolated from the parasite excretory-secretory products in sufficient quantities for use as an antigen for the serodiagnosis of humna fasciolosis. The methods illustrated in this chapter willexplain the isolation of cysteine proteases from E hepatica excretory-secretory products by gel filtration and anion exchange chromatography, and their subsequent characterisation and employment in an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-fasciola antibodies int he serum of infected humans
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