Identification of lipoprotein MslA as a neoteric virulence factor of Mycoplasma gallisepticum

Amer Soc Microbiology
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Journal Article
Infection And Immunity, 2010, 78 (8), pp. 3475 - 3483
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Many lipoproteins are expressed on the surfaces of mycoplasmas, and some have been implicated as playing roles in pathogenesis. Family 2 lipoproteins of Mycoplasma plleumoniae have a conserved "mycoplasma liM poprotein X" central domain and a "mycoplasma lipoprotein 10" CMterminal domain and are differentially expressed in response to environmental conditions. Homologues of family 2 lipoproteins are Mycoplasma specific and include the lipoprotein of Mycoplasma gaIlisepticum, encoded by the MGA0674 gene. Comparative transcriptomic analysis of the M. gaUisepticum live attenuated vaccine strain F and the virulent strain Rio"",, reported in this study, indicated that MGA0674 is one of several differentially expressed genes. The MGA0674M encoded lipoprotein is a proteolytically processed, immunogenic, TXM114 detergentMphase protein which apM pears to have antigenic divergence between field strains R10w and S6. We examined the virulence of an R10w AMGA0674 mutant (PIH9) in vivo and observed reduced recovery and attenuated virulence in the tracheas of experimentally infected chickens. The virulence of two additional R10w AMGA0674 mutants, 2162 and 2204, was assessed in a second ill vivo virulence experiment. These mutants exhibited partial to complete attenuation in vivo, but recovery was observed more frequently. Since only Mycoplasma species harbor homologues of MGA0674, the gene product has been renamed "AlycoplasmaMspecific lipoprotein A" (MslA). Collectively, these data indicate that MslA is an immunogenic lipoprotein exhibiting reduced expression in an attenuated strain and plays a role in M. gallisepticum virulence.
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