The preparation and use of biotinylated trypsin in western blotting for the detection of trypsin inhibitory proteins

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Journal Article
Analytical Biochemistry, 1994, 222 (1), pp. 34 - 43
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Methods were developed for the preparation of biotinylated trypsin of high specific activity. This was used as a probe for the detection of serine proteinase inhibitory proteins which had been separated by sodium dodecyl sulfate-polyacrylamide gradient s
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