Applying microfluidics for industrial production of stem cells

Ding, Lin

Applying microfluidics for industrial production of stem cells

Ding, Lin

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Publication Type:: Thesis
Issue Date:: 2022

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Field	Value	Language
dc.contributor.author	Ding, Lin
dc.date.accessioned	2023-07-14T01:56:01Z
dc.date.available	2023-07-14T01:56:01Z
dc.date.issued	2022
dc.identifier.uri	http://hdl.handle.net/10453/171499
dc.description	University of Technology Sydney. Faculty of Engineering and Information Technology.	en_US.UTF-8
dc.description.abstract	Stem cells have immense potential in the fields of regenerative medicine, drug discovery, and cellular agriculture. As our understanding of these cells deepens, the demand for them is increasing rapidly. However, the current production process is inadequate to meet this high demand, leading to low yields and poor product quality. This inconsistency in quality results in unstable therapeutic outcomes and clinical trial failures, leading to increased costs of stem cell products. Microfluidic technology presents a promising solution to these challenges. It utilizes small channels to control the flow of liquids precisely and has been found to have potential in a range of biotechnology applications due to the precision handling of fluid flow. microfluidic devices provide better outcomes such as high processing efficiency, low-cost, and clogging-free separation. This thesis showcases the application of microfluidic devices in the stem cell industry including a high throughput microfluidic droplet generator for producing dissolvable and edible microcarriers for the cultivated meat industry, a 3D printed microfluidic system for detaching stem cells from current commercial microcarriers, and a micromixer for homogeneously mixing cryoprotectant and stem cells, replacing current industrial bulk mixing methods. The thesis also provides potential future works related to microfluidic applications in the field.	en_US.UTF-8
dc.format	Thesis (PhD)
dc.language.iso	en_US	en_US.UTF-8
dc.relation	https://opus.lib.uts.edu.au/bitstream/10453/171499/2/02whole.pdf
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	© 2022 Lin Ding
dc.rights	au.edu.uts.lib/cph
dc.rights	The author owns the copyright in this thesis including all reproduction and reuse rights for the work. The work may not be altered without the permission of the copyright owner. Attribution is essential when quoting or paraphrasing from this thesis.
dc.title	Applying microfluidics for industrial production of stem cells	en_US.UTF-8
dc.type	Thesis
utslib.copyright.status	open_access	*

Abstract:

Stem cells have immense potential in the fields of regenerative medicine, drug discovery, and cellular agriculture. As our understanding of these cells deepens, the demand for them is increasing rapidly. However, the current production process is inadequate to meet this high demand, leading to low yields and poor product quality. This inconsistency in quality results in unstable therapeutic outcomes and clinical trial failures, leading to increased costs of stem cell products. Microfluidic technology presents a promising solution to these challenges. It utilizes small channels to control the flow of liquids precisely and has been found to have potential in a range of biotechnology applications due to the precision handling of fluid flow. microfluidic devices provide better outcomes such as high processing efficiency, low-cost, and clogging-free separation. This thesis showcases the application of microfluidic devices in the stem cell industry including a high throughput microfluidic droplet generator for producing dissolvable and edible microcarriers for the cultivated meat industry, a 3D printed microfluidic system for detaching stem cells from current commercial microcarriers, and a micromixer for homogeneously mixing cryoprotectant and stem cells, replacing current industrial bulk mixing methods. The thesis also provides potential future works related to microfluidic applications in the field.

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http://hdl.handle.net/10453/171499