The relationship between nucleoside triphosphate hydrolase (NTPase) isoform and Toxoplasma strain virulence in rat and human toxoplasmosis

Johnson, M; Broady, K; Angelici, MC; Johnson, A

The relationship between nucleoside triphosphate hydrolase (NTPase) isoform and Toxoplasma strain virulence in rat and human toxoplasmosis

Johnson, M Broady, K Angelici, MC Johnson, A

Permalink

Publication Type:: Journal Article
Citation:: Microbes and Infection, 2003, 5 (9), pp. 797 - 806
Issue Date:: 2003-01-01

Closed Access

	Filename	Description	Size
	2003001012.pdf		166.74 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Johnson, M	en_US
dc.contributor.author	Broady, K	en_US
dc.contributor.author	Angelici, MC	en_US
dc.contributor.author	Johnson, A	en_US
dc.date.issued	2003-01-01	en_US
dc.identifier.citation	Microbes and Infection, 2003, 5 (9), pp. 797 - 806	en_US
dc.identifier.issn	1286-4579	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3719
dc.description.abstract	Avirulent strains of Toxoplasma gondii possess only the nucleoside triphosphate hydrolase II (NTPaseII) isoform, whilst virulent strains possess both NTPaseI and NTPaseII. To determine if it is possible to identify the infective strain type (virulent or avirulent) in T. gondii infections by serological methods, we developed isoform-specific peptide ELISAs from the NTPaseI and NTPaseII antigens of T. gondii. When rats were immunized with either recombinant NTPaseI or NTPaseII, the ELISA could differentially identify antibody reactivity to each NTPase isoform. This ELISA was then used to test six groups of rats that were infected with either one of three virulent (RH, P or Ent) or three avirulent (Me49, C or TPR) strains of T. gondii. No differential antibody reactivity was detected by either whole recNTPase ELISA or peptide ELISA in the sera of rats, whether infected by virulent or avirulent strains of T. gondii. We also studied a panel of human sera from patients infected with known laboratory strains of T. gondii or naturally infected patients where the parasite was isolated and its virulence determined in mice. Differential reactivity to whole recNTPase isoforms was detected in some human sera, but this reactivity was not detected by the isoform-specific peptide ELISAs. Although the NTPase peptides do exhibit differential antibody reactivity, this is not correlated with the virulence status of the infecting strain. © 2003 Éditions scientifiques et médicales Elsevier SAS. All rights reserved.	en_US
dc.relation.ispartof	Microbes and Infection	en_US
dc.relation.isbasedon	10.1016/S1286-4579(03)00148-5	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Rats, Inbred Strains	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Rats	en_US
dc.subject.mesh	Toxoplasma	en_US
dc.subject.mesh	Toxoplasmosis	en_US
dc.subject.mesh	Acid Anhydride Hydrolases	en_US
dc.subject.mesh	Nucleoside-Triphosphatase	en_US
dc.subject.mesh	Isoenzymes	en_US
dc.subject.mesh	Recombinant Proteins	en_US
dc.subject.mesh	Antibodies, Protozoan	en_US
dc.subject.mesh	Enzyme-Linked Immunosorbent Assay	en_US
dc.subject.mesh	Immunization	en_US
dc.subject.mesh	Virulence	en_US
dc.title	The relationship between nucleoside triphosphate hydrolase (NTPase) isoform and Toxoplasma strain virulence in rat and human toxoplasmosis	en_US
dc.type	Journal Article
utslib.citation.volume	9	en_US
utslib.citation.volume	5	en_US
utslib.for	110705 Humoural Immunology and Immunochemistry	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1107 Immunology	en_US
utslib.for	1108 Medical Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	9	en_US
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

Avirulent strains of Toxoplasma gondii possess only the nucleoside triphosphate hydrolase II (NTPaseII) isoform, whilst virulent strains possess both NTPaseI and NTPaseII. To determine if it is possible to identify the infective strain type (virulent or avirulent) in T. gondii infections by serological methods, we developed isoform-specific peptide ELISAs from the NTPaseI and NTPaseII antigens of T. gondii. When rats were immunized with either recombinant NTPaseI or NTPaseII, the ELISA could differentially identify antibody reactivity to each NTPase isoform. This ELISA was then used to test six groups of rats that were infected with either one of three virulent (RH, P or Ent) or three avirulent (Me49, C or TPR) strains of T. gondii. No differential antibody reactivity was detected by either whole recNTPase ELISA or peptide ELISA in the sera of rats, whether infected by virulent or avirulent strains of T. gondii. We also studied a panel of human sera from patients infected with known laboratory strains of T. gondii or naturally infected patients where the parasite was isolated and its virulence determined in mice. Differential reactivity to whole recNTPase isoforms was detected in some human sera, but this reactivity was not detected by the isoform-specific peptide ELISAs. Although the NTPase peptides do exhibit differential antibody reactivity, this is not correlated with the virulence status of the infecting strain. © 2003 Éditions scientifiques et médicales Elsevier SAS. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/3719