Performance characteristics and optimisation of cut-off values of two enzyme-linked immunosorbent assays for the detection of antibodies to Neospora caninum in the serum of cattle

Hall, CA; Reichel, MP; Ellis, JT

Performance characteristics and optimisation of cut-off values of two enzyme-linked immunosorbent assays for the detection of antibodies to Neospora caninum in the serum of cattle

Hall, CA Reichel, MP

Ellis, JT

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Publication Type:: Journal Article
Citation:: Veterinary Parasitology, 2006, 140 (1-2), pp. 61 - 68
Issue Date:: 2006-08-31

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Field	Value	Language
dc.contributor.author	Hall, CA	en_US
dc.contributor.author	Reichel, MP https://orcid.org/0000-0002-9792-8336	en_US
dc.contributor.author	Ellis, JT https://orcid.org/0000-0001-7328-4831	en_US
dc.date.available	2006-03-13	en_US
dc.date.issued	2006-08-31	en_US
dc.identifier.citation	Veterinary Parasitology, 2006, 140 (1-2), pp. 61 - 68	en_US
dc.identifier.issn	0304-4017	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3948
dc.description.abstract	Aim: To determine the performance characteristics of two enzyme-linked immunosorbent assays (ELISAs) manufactured by Institut Pourquier (IP) for the detection of antibodies against Neospora caninum in bovine sera. Methods: Sera from 526 cattle were assayed in two ELISAs (IP) for the detection of anti-N. caninum antibodies. Results from a further ELISA (IDEXX) were used to provide the "gold standard" N. caninum infection status of the cattle and the ELISA results assessed by two-graph receiver operating characteristic (TG-ROC) analysis. Results: TG-ROC analysis suggested changes to one of the IP ELISA protocols, arriving at a cut-off threshold that was different to the one recommended by the manufacturer. With that change, both of the ELISAs performed with high sensitivity and specificity (in excess of 98%) for bovine sera. Conclusions: The analysis of the two IP ELISAs when used on individual bovine sera demonstrated high sensitivity and specificity. TG-ROC analyses optimised the cut-off point suggested by the manufacturer for one of these commercial diagnostic assays and found agreement with the manufacturer's cut-off regarding the other assay. This will help with the accurate identification of infected animals and thereby contributing to the control of neosporosis. © 2006 Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	Veterinary Parasitology	en_US
dc.relation.isbasedon	10.1016/j.vetpar.2006.03.016	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Cattle	en_US
dc.subject.mesh	Neospora	en_US
dc.subject.mesh	Coccidiosis	en_US
dc.subject.mesh	Cattle Diseases	en_US
dc.subject.mesh	Antibodies, Protozoan	en_US
dc.subject.mesh	Enzyme-Linked Immunosorbent Assay	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	ROC Curve	en_US
dc.subject.mesh	Reference Standards	en_US
dc.subject.mesh	New South Wales	en_US
dc.subject.mesh	Female	en_US
dc.title	Performance characteristics and optimisation of cut-off values of two enzyme-linked immunosorbent assays for the detection of antibodies to Neospora caninum in the serum of cattle	en_US
dc.type	Journal Article
utslib.citation.volume	1-2	en_US
utslib.citation.volume	140	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0704 Fisheries Sciences	en_US
utslib.for	0707 Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Business
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	1-2	en_US
pubs.publication-status	Published	en_US
pubs.volume	140	en_US

Abstract:

Aim: To determine the performance characteristics of two enzyme-linked immunosorbent assays (ELISAs) manufactured by Institut Pourquier (IP) for the detection of antibodies against Neospora caninum in bovine sera. Methods: Sera from 526 cattle were assayed in two ELISAs (IP) for the detection of anti-N. caninum antibodies. Results from a further ELISA (IDEXX) were used to provide the "gold standard" N. caninum infection status of the cattle and the ELISA results assessed by two-graph receiver operating characteristic (TG-ROC) analysis. Results: TG-ROC analysis suggested changes to one of the IP ELISA protocols, arriving at a cut-off threshold that was different to the one recommended by the manufacturer. With that change, both of the ELISAs performed with high sensitivity and specificity (in excess of 98%) for bovine sera. Conclusions: The analysis of the two IP ELISAs when used on individual bovine sera demonstrated high sensitivity and specificity. TG-ROC analyses optimised the cut-off point suggested by the manufacturer for one of these commercial diagnostic assays and found agreement with the manufacturer's cut-off regarding the other assay. This will help with the accurate identification of infected animals and thereby contributing to the control of neosporosis. © 2006 Elsevier B.V. All rights reserved.

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http://hdl.handle.net/10453/3948