Unexpected photobleaching of Alexa 488 in a fixed bacterial sample during 2-photon excitation

Peters, PC; Thoni, C; Harry, EJ

Unexpected photobleaching of Alexa 488 in a fixed bacterial sample during 2-photon excitation

Peters, PC Thoni, C Harry, EJ

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Publication Type:: Journal Article
Citation:: Biotechnic and Histochemistry, 2006, 81 (2-3), pp. 105 - 106
Issue Date:: 2006-03-01

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Field	Value	Language
dc.contributor.author	Peters, PC	en_US
dc.contributor.author	Thoni, C	en_US
dc.contributor.author	Harry, EJ https://orcid.org/0000-0003-0858-9473	en_US
dc.date.issued	2006-03-01	en_US
dc.identifier.citation	Biotechnic and Histochemistry, 2006, 81 (2-3), pp. 105 - 106	en_US
dc.identifier.issn	1052-0295	en_US
dc.identifier.uri	http://hdl.handle.net/10453/4352
dc.description.abstract	A sample of fixed bacterial cells was examined by immunofluorescence microscopy using an Alexa 488 conjugated secondary antibody for visualization. Excitation using visible light confirmed the expected photostability of this fluorophore; however, when using 2-photon excitation, Alexa 488 was rapidly and substantially photobleached. The unexpected instability of Alexa 488 under certain conditions may have deleterious consequences if not anticipated and accommodated in experimental protocols.	en_US
dc.relation.ispartof	Biotechnic and Histochemistry	en_US
dc.relation.isbasedon	10.1080/10520290600869355	en_US
dc.subject.classification	Pathology	en_US
dc.subject.mesh	Bacillus subtilis	en_US
dc.subject.mesh	Hydrazines	en_US
dc.subject.mesh	Microscopy, Fluorescence	en_US
dc.subject.mesh	Staining and Labeling	en_US
dc.subject.mesh	Photobleaching	en_US
dc.title	Unexpected photobleaching of Alexa 488 in a fixed bacterial sample during 2-photon excitation	en_US
dc.type	Journal Article
utslib.citation.volume	2-3	en_US
utslib.citation.volume	81	en_US
utslib.for	1108 Medical Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	2-3	en_US
pubs.publication-status	Published	en_US
pubs.volume	81	en_US

Abstract:

A sample of fixed bacterial cells was examined by immunofluorescence microscopy using an Alexa 488 conjugated secondary antibody for visualization. Excitation using visible light confirmed the expected photostability of this fluorophore; however, when using 2-photon excitation, Alexa 488 was rapidly and substantially photobleached. The unexpected instability of Alexa 488 under certain conditions may have deleterious consequences if not anticipated and accommodated in experimental protocols.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/4352