Quantitative elemental bio-imaging of Mn, Fe, Cu and Zn in 6-hydroxydopamine induced Parkinsonism mouse models

Hare, D; Reedy, B; Grimm, R; Wilkins, S; Volitakis, I; George, JL; Cherny, RA; Bush, AI; Finkelstein, DI; Doble, P

Quantitative elemental bio-imaging of Mn, Fe, Cu and Zn in 6-hydroxydopamine induced Parkinsonism mouse models

Hare, D

Reedy, B

Grimm, R Wilkins, S Volitakis, I George, JL Cherny, RA Bush, AI Finkelstein, DI Doble, P

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Publication Type:: Journal Article
Citation:: Metallomics, 2009, 1 (1), pp. 53 - 58
Issue Date:: 2009-01-01

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Field	Value	Language
dc.contributor.author	Hare, D https://orcid.org/0000-0002-5922-7643	en_US
dc.contributor.author	Reedy, B https://orcid.org/0000-0003-1797-8091	en_US
dc.contributor.author	Grimm, R	en_US
dc.contributor.author	Wilkins, S	en_US
dc.contributor.author	Volitakis, I	en_US
dc.contributor.author	George, JL	en_US
dc.contributor.author	Cherny, RA	en_US
dc.contributor.author	Bush, AI	en_US
dc.contributor.author	Finkelstein, DI	en_US
dc.contributor.author	Doble, P https://orcid.org/0000-0002-8472-1301	en_US
dc.date.issued	2009-01-01	en_US
dc.identifier.citation	Metallomics, 2009, 1 (1), pp. 53 - 58	en_US
dc.identifier.issn	1756-5901	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8417
dc.description.abstract	This study demonstrates the application of quantitative elemental bio-imaging for the determination of the distribution Cu, Mn, Fe and Zn in Parkinsonism mouse model brains. Elevated concentrations of these metals within the substantia nigra (SN) are suspected to play a role on the development of Parkinson's disease. Elemental bio-imaging employs laser ablation inductively coupled mass spectrometry (LA-ICP-MS) to construct images of trace element distribution. Quantitative data was produced by ablating the standard tissue sections and recording the mean signal intensity calibrated against multi level matrix matched tissue standards. The concentrations of Fe within the substantia nigra of the lesioned animals increased significantly when compared against control animals. Furthermore, the data was compared against solution nebulisation ICP-MS in which the whole substantia nigra was excised. The trends were the same for both methods; however the elemental bio-imaging method returned significantly higher concentrations. This was caused by dilution from inclusion of surrounding tissue of the SN during the excision procedure. © The Royal Society of Chemistry 2009.	en_US
dc.relation.ispartof	Metallomics	en_US
dc.relation.isbasedon	10.1039/b816188g	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.title	Quantitative elemental bio-imaging of Mn, Fe, Cu and Zn in 6-hydroxydopamine induced Parkinsonism mouse models	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.for	039902 Forensic Chemistry	en_US
utslib.for	03 Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	1	en_US

Abstract:

This study demonstrates the application of quantitative elemental bio-imaging for the determination of the distribution Cu, Mn, Fe and Zn in Parkinsonism mouse model brains. Elevated concentrations of these metals within the substantia nigra (SN) are suspected to play a role on the development of Parkinson's disease. Elemental bio-imaging employs laser ablation inductively coupled mass spectrometry (LA-ICP-MS) to construct images of trace element distribution. Quantitative data was produced by ablating the standard tissue sections and recording the mean signal intensity calibrated against multi level matrix matched tissue standards. The concentrations of Fe within the substantia nigra of the lesioned animals increased significantly when compared against control animals. Furthermore, the data was compared against solution nebulisation ICP-MS in which the whole substantia nigra was excised. The trends were the same for both methods; however the elemental bio-imaging method returned significantly higher concentrations. This was caused by dilution from inclusion of surrounding tissue of the SN during the excision procedure. © The Royal Society of Chemistry 2009.

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http://hdl.handle.net/10453/8417