Venomics: Unravelling the complexity of animal venoms with mass spectrometry

Escoubas, P; Quinton, L; Nicholson, GM

Venomics: Unravelling the complexity of animal venoms with mass spectrometry

Escoubas, P Quinton, L Nicholson, GM

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Publication Type:: Journal Article
Citation:: Journal of Mass Spectrometry, 2008, 43 (3), pp. 279 - 295
Issue Date:: 2008-03-01

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Field	Value	Language
dc.contributor.author	Escoubas, P	en_US
dc.contributor.author	Quinton, L	en_US
dc.contributor.author	Nicholson, GM https://orcid.org/0000-0002-4277-4296	en_US
dc.date.issued	2008-03-01	en_US
dc.identifier.citation	Journal of Mass Spectrometry, 2008, 43 (3), pp. 279 - 295	en_US
dc.identifier.issn	1076-5174	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8451
dc.description.abstract	Animal venoms and toxins are now recognized as major sources of bioactive molecules that may be tomorrow's new drug leads. Their complexity and their potential as drug sources have been demonstrated by application of modern analytical technologies, which have revealed venoms to be vast peptide combinatorial libraries. Structural as well as pharmacological diversity is immense, and mass spectrometry is now one of the major investigative tools for the structural investigation of venom components. Recent advances in its use in the study of venom and toxins are reviewed. The application of mass spectrometry techniques to peptide toxin sequence determination by de novo sequencing is discussed in detail, in the light of the search for novel analgesic drugs. We also present the combined application of LC-MALDI separation with mass fingerprinting and ISD fragmentation for the determination of structural and pharmacological classes of peptides in complex spider venoms. This approach now serves as the basis for the full investigation of complex spider venom proteomes, in combination with cDNA analysis. Copyright © 2008 John Wiley & Sons, Ltd.	en_US
dc.relation.ispartof	Journal of Mass Spectrometry	en_US
dc.relation.isbasedon	10.1002/jms.1389	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Venoms	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Pain	en_US
dc.subject.mesh	Spider Venoms	en_US
dc.subject.mesh	Chromatography, Liquid	en_US
dc.subject.mesh	Peptide Mapping	en_US
dc.subject.mesh	Sequence Analysis, Protein	en_US
dc.subject.mesh	Proteomics	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Mass Spectrometry	en_US
dc.title	Venomics: Unravelling the complexity of animal venoms with mass spectrometry	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	43	en_US
utslib.for	0399 Other Chemical Sciences	en_US
utslib.for	03 Chemical Sciences	en_US
dc.location.activity	ISI:000254270900001	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	43	en_US

Abstract:

Animal venoms and toxins are now recognized as major sources of bioactive molecules that may be tomorrow's new drug leads. Their complexity and their potential as drug sources have been demonstrated by application of modern analytical technologies, which have revealed venoms to be vast peptide combinatorial libraries. Structural as well as pharmacological diversity is immense, and mass spectrometry is now one of the major investigative tools for the structural investigation of venom components. Recent advances in its use in the study of venom and toxins are reviewed. The application of mass spectrometry techniques to peptide toxin sequence determination by de novo sequencing is discussed in detail, in the light of the search for novel analgesic drugs. We also present the combined application of LC-MALDI separation with mass fingerprinting and ISD fragmentation for the determination of structural and pharmacological classes of peptides in complex spider venoms. This approach now serves as the basis for the full investigation of complex spider venom proteomes, in combination with cDNA analysis. Copyright © 2008 John Wiley & Sons, Ltd.

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http://hdl.handle.net/10453/8451