Multiplex PCR allows simultaneous detection of pathogens in ships' ballast water

Aridgides, LJ; Doblin, MA; Berke, T; Dobbs, FC; Matson, DO; Drake, LA

Multiplex PCR allows simultaneous detection of pathogens in ships' ballast water

Aridgides, LJ Doblin, MA

Berke, T Dobbs, FC Matson, DO Drake, LA

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Publication Type:: Journal Article
Citation:: Marine Pollution Bulletin, 2004, 48 (11-12), pp. 1096 - 1101
Issue Date:: 2004-06-01

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Field	Value	Language
dc.contributor.author	Aridgides, LJ	en_US
dc.contributor.author	Doblin, MA https://orcid.org/0000-0001-8750-3433	en_US
dc.contributor.author	Berke, T	en_US
dc.contributor.author	Dobbs, FC	en_US
dc.contributor.author	Matson, DO	en_US
dc.contributor.author	Drake, LA	en_US
dc.date.issued	2004-06-01	en_US
dc.identifier.citation	Marine Pollution Bulletin, 2004, 48 (11-12), pp. 1096 - 1101	en_US
dc.identifier.issn	0025-326X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8628
dc.description.abstract	There is enormous potential for global transfer of microorganisms, including pathogens, in ships' ballast water. We contend that a major advancement in the study of ballast-water microorganisms in particular, and of aquatic pathogens in general, will be expedited sample analysis, such as provided by the elegant technology of DNA microarrays. In order to use DNA microarrays, however, one must establish the appropriate conditions to bind target sequences in samples to multiple probes on the microarrays. We conducted proof-of-concept experiments to optimize simultaneous detection of multiple microorganisms using polymerase chain reaction (PCR) and Southern hybridization. We chose three target organisms, all potentially found in ballast water: a calicivirus, the bacterium Vibrio cholerae, and the photosynthetic protist Aureococcus anophagefferens. Here, we show simultaneous detection of multiple pathogens is possible, a result supporting the promising future use of microarrays for simultaneous detection of pathogens in ballast water. © 2003 Elsevier Ltd. All rights reserved.	en_US
dc.relation.ispartof	Marine Pollution Bulletin	en_US
dc.relation.isbasedon	10.1016/j.marpolbul.2003.12.017	en_US
dc.subject.classification	Marine Biology & Hydrobiology	en_US
dc.subject.mesh	Vibrio cholerae	en_US
dc.subject.mesh	Caliciviridae	en_US
dc.subject.mesh	Oligonucleotides	en_US
dc.subject.mesh	Blotting, Southern	en_US
dc.subject.mesh	Oligonucleotide Array Sequence Analysis	en_US
dc.subject.mesh	Feasibility Studies	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Reverse Transcriptase Polymerase Chain Reaction	en_US
dc.subject.mesh	Seawater	en_US
dc.subject.mesh	Environmental Monitoring	en_US
dc.subject.mesh	Ships	en_US
dc.subject.mesh	Chrysophyta	en_US
dc.title	Multiplex PCR allows simultaneous detection of pathogens in ships' ballast water	en_US
dc.type	Journal Article
utslib.citation.volume	11-12	en_US
utslib.citation.volume	48	en_US
utslib.for	060205 Marine and Estuarine Ecology (incl. Marine Ichthyology)	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	11-12	en_US
pubs.publication-status	Published	en_US
pubs.volume	48	en_US

Abstract:

There is enormous potential for global transfer of microorganisms, including pathogens, in ships' ballast water. We contend that a major advancement in the study of ballast-water microorganisms in particular, and of aquatic pathogens in general, will be expedited sample analysis, such as provided by the elegant technology of DNA microarrays. In order to use DNA microarrays, however, one must establish the appropriate conditions to bind target sequences in samples to multiple probes on the microarrays. We conducted proof-of-concept experiments to optimize simultaneous detection of multiple microorganisms using polymerase chain reaction (PCR) and Southern hybridization. We chose three target organisms, all potentially found in ballast water: a calicivirus, the bacterium Vibrio cholerae, and the photosynthetic protist Aureococcus anophagefferens. Here, we show simultaneous detection of multiple pathogens is possible, a result supporting the promising future use of microarrays for simultaneous detection of pathogens in ballast water. © 2003 Elsevier Ltd. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/8628