Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms

Barken, KB; Pamp, SJ; Yang, L; Gjermansen, M; Bertrand, JJ; Klausen, M; Givskov, M; Whitchurch, CB; Engel, JN; Tolker-Nielsen, T

Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms

Barken, KB Pamp, SJ Yang, L Gjermansen, M Bertrand, JJ Klausen, M Givskov, M Whitchurch, CB

Engel, JN Tolker-Nielsen, T

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Publication Type:: Journal Article
Citation:: Environmental Microbiology, 2008, 10 (9), pp. 2331 - 2343
Issue Date:: 2008-09-01

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Field	Value	Language
dc.contributor.author	Barken, KB	en_US
dc.contributor.author	Pamp, SJ	en_US
dc.contributor.author	Yang, L	en_US
dc.contributor.author	Gjermansen, M	en_US
dc.contributor.author	Bertrand, JJ	en_US
dc.contributor.author	Klausen, M	en_US
dc.contributor.author	Givskov, M	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Engel, JN	en_US
dc.contributor.author	Tolker-Nielsen, T	en_US
dc.date.issued	2008-09-01	en_US
dc.identifier.citation	Environmental Microbiology, 2008, 10 (9), pp. 2331 - 2343	en_US
dc.identifier.issn	1462-2912	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8814
dc.description.abstract	When grown as a biofilm in laboratory flow chambers Pseudomonas aeruginosa can develop mushroom-shaped multicellular structures consisting of distinct subpopulations in the cap and stalk portions. We have previously presented evidence that formation of the cap portion of the mushroom-shaped structures in P. aeruginosa biofilms occurs via bacterial migration and depends on type IV pili (Mol Microbiol 50: 61-68). In the present study we examine additional factors involved in the formation of this multicellular substructure. While pilA mutants, lacking type IV pili, are deficient in mushroom cap formation, pilH and chpA mutants, which are inactivated in the type IV pili-linked chemosensory system, showed only minor defects in cap formation. On the contrary, fliM mutants, which are non-flagellated, and cheY mutants, which are inactivated in the flagellum-linked chemotaxis system, were largely deficient in cap formation. Experiments involving DNase treatment of developing biofilms provided evidence that extracellular DNA plays a role in cap formation. Moreover, mutants that are deficient in quorum sensing-controlled DNA release formed microcolonies upon which wild-type bacteria could not form caps. These results constitute evidence that type IV pili, flagellum-mediated motility and quorum sensing-controlled DNA release are involved in the formation of mature multicellular structures in P. aeruginosa biofilms. © 2008 The Authors.	en_US
dc.relation.ispartof	Environmental Microbiology	en_US
dc.relation.isbasedon	10.1111/j.1462-2920.2008.01658.x	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Fimbriae, Bacterial	en_US
dc.subject.mesh	Flagella	en_US
dc.subject.mesh	Extracellular Matrix	en_US
dc.subject.mesh	Biofilms	en_US
dc.subject.mesh	Pseudomonas aeruginosa	en_US
dc.subject.mesh	Fimbriae Proteins	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	Microscopy, Confocal	en_US
dc.subject.mesh	Genetic Complementation Test	en_US
dc.subject.mesh	Bacterial Adhesion	en_US
dc.subject.mesh	Chemotaxis	en_US
dc.subject.mesh	Genotype	en_US
dc.subject.mesh	Genes, Bacterial	en_US
dc.subject.mesh	Quorum Sensing	en_US
dc.title	Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms	en_US
dc.type	Journal Article
utslib.citation.volume	9	en_US
utslib.citation.volume	10	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0603 Evolutionary Biology	en_US
dc.location.activity	ISI:000258203500013	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	9	en_US
pubs.publication-status	Published	en_US
pubs.volume	10	en_US

Abstract:

When grown as a biofilm in laboratory flow chambers Pseudomonas aeruginosa can develop mushroom-shaped multicellular structures consisting of distinct subpopulations in the cap and stalk portions. We have previously presented evidence that formation of the cap portion of the mushroom-shaped structures in P. aeruginosa biofilms occurs via bacterial migration and depends on type IV pili (Mol Microbiol 50: 61-68). In the present study we examine additional factors involved in the formation of this multicellular substructure. While pilA mutants, lacking type IV pili, are deficient in mushroom cap formation, pilH and chpA mutants, which are inactivated in the type IV pili-linked chemosensory system, showed only minor defects in cap formation. On the contrary, fliM mutants, which are non-flagellated, and cheY mutants, which are inactivated in the flagellum-linked chemotaxis system, were largely deficient in cap formation. Experiments involving DNase treatment of developing biofilms provided evidence that extracellular DNA plays a role in cap formation. Moreover, mutants that are deficient in quorum sensing-controlled DNA release formed microcolonies upon which wild-type bacteria could not form caps. These results constitute evidence that type IV pili, flagellum-mediated motility and quorum sensing-controlled DNA release are involved in the formation of mature multicellular structures in P. aeruginosa biofilms. © 2008 The Authors.

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http://hdl.handle.net/10453/8814