Blood protein purification and simultaneous removal of nonenveloped viruses using tangential-flow preparative electrophoresis.
- Publication Type:
- Journal Article
- Citation:
- Electrophoresis, 2005, 26 (1), pp. 28 - 34
- Issue Date:
- 2005-01
Closed Access
| Filename | Description | Size | |||
|---|---|---|---|---|---|
 | 2008005622OK.pdf | 365.65 kB |
Copyright Clearance Process
- Recently Added
- In Progress
- Closed Access
This item is closed access and not available.
Gradiflow is new technology allowing purification of important blood proteins from viral contaminated plasma. Protein purification is based on unique scalable tangential-flow preparative electrophoresis, and is distinct from current technology because protein purification and virus removal are performed in the same step. This one-step removal and purification exploits both the size and charge of target proteins. The medically important blood proteins, immunoglobulin G (IgG) and alpha-1-antitrypsin, were chosen to demonstrate the ability of this process to purify proteins from contaminated plasma. Clearance factors achieved by infectivity assays and polymerase chain reaction (PCR) that meet regulatory requirements demonstrated removal of canine parvovirus (CPV). CPV is a model virus for pathogenic nonenveloped viruses, including parvovirus B19, not adequately removed or inactivated by most processes currently in practice. The recovery of proteins from plasma with high purity, recovery, and function, while simultaneously removing viruses, provides blood products with a level of purity compatible with clinical use more quickly and cheaply than available techniques.
Please use this identifier to cite or link to this item: