Isolation and characterisation of cathepsin L1 protease from Fasciola hepatica excretory-secretory products for serodiagnosis of human fasciolosis

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dc.contributor.author O'Neill, S
dc.contributor.author Mulcahy, G
dc.contributor.author Dalton, JP
dc.contributor.editor Adley, CC
dc.date.accessioned 2010-06-16T04:55:46Z
dc.date.issued 2006-01
dc.identifier.citation Methods in Biotechnology: Food Borne Pathogens, 2006, 1, pp. 191 - 202
dc.identifier.isbn 1-59259-990-7
dc.identifier.other B1 en_US
dc.identifier.uri http://hdl.handle.net/10453/11658
dc.description.abstract The major antigens secreted by the parasite Fasciola hepatica are cathepsin-L cysteine proteases. The enzymes can be isolated from the parasite excretory-secretory products in sufficient quantities for use as an antigen for the serodiagnosis of humna fasciolosis. The methods illustrated in this chapter willexplain the isolation of cysteine proteases from E hepatica excretory-secretory products by gel filtration and anion exchange chromatography, and their subsequent characterisation and employment in an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-fasciola antibodies int he serum of infected humans
dc.publisher Humana Press Inc.
dc.title Isolation and characterisation of cathepsin L1 protease from Fasciola hepatica excretory-secretory products for serodiagnosis of human fasciolosis
dc.type Chapter
dc.parent Methods in Biotechnology: Food Borne Pathogens
dc.journal.number en_US
dc.publocation New Jersey, USA en_US
dc.publocation Piscataway, USA
dc.publocation New Jersey, USA
dc.identifier.startpage 191 en_US
dc.identifier.endpage 202 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.conference International Conference on Industrial Informatics
dc.for 060107 Enzymes
dc.for 110803 Medical Parasitology
dc.personcode 030896
dc.percentage 50 en_US
dc.classification.name Medical Parasitology en_US
dc.classification.type FOR-08 en_US
dc.edition 1 en_US
dc.edition 1
dc.custom en_US
dc.date.activity en_US
dc.date.activity 2005-08-10
dc.location.activity en_US
dc.location.activity Perth, Aust
dc.description.keywords Cathepsin-L proteases
dc.description.keywords gel filtration
dc.description.keywords anion exchange chromatography
dc.description.keywords ELISA
dc.description.keywords in vitro culture
dc.description.keywords parasites
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history Closed (ID: 3)


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