Extracellular matrix remodelling during cell adhesion monitored by the quartz crystal microbalance

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dc.contributor.author Lord, MS
dc.contributor.author Modin, C
dc.contributor.author Foss, M
dc.contributor.author Duch, M
dc.contributor.author Simmons, A
dc.contributor.author Pedersen, FS
dc.contributor.author Besenbacher, F
dc.contributor.author Milthorpe, BK
dc.date.accessioned 2010-07-15T07:27:20Z
dc.date.issued 2008-06
dc.identifier.citation Biomaterials, 2008, 29 (17), pp. 2581 - 2587
dc.identifier.issn 0142-9612
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/12812
dc.description.abstract A cell's ability to remodel adsorbed protein layers on surfaces is influenced by the nature of the protein layer itself. Remodelling is often required to accomplish cellular adhesion and extracellular matrix formation which forms the basis for cell spreading, increased adhesion and expression of different phenotypes. The adhesion of NIH3T3 (EGFP) fibroblasts to serum protein (albumin or fibronectin) precoated tantalum (Ta) and oxidised polystyrene (PS ox) surfaces was examined using the quartz crystal microbalance with dissipation (QCM-D) monitoring and fluorescence microscopy. The cells were either untreated or treated with cycloheximide to examine the contribution of endogenous protein production during cell adhesion to the QCM-D response over a period of 2 h. Following adsorption of albumin onto Ta and PS ox there was no difference detected between the response to seeding untreated and cycloheximide treated cells. The QCM-D was able to detect differences in the untreated cellular responses to fibronectin versus serum precoated Ta and PS ox substrates, while cycloheximide treatment of the cells produced the same QCM-D response for fibronectin and serum precoatings on each of the materials. This confirmed that the process of matrix remodelling by the cells is dependent on the underlying substrate and the preadsorbed proteins and that the QCM-D response is dominated by changes in the underlying protein layer. Changes in dissipation correspond to the development of the actin cytoskeleton as visualised by actin staining. © 2008 Elsevier Ltd. All rights reserved.
dc.language eng
dc.relation.isbasedon 10.1016/j.biomaterials.2008.03.002
dc.subject Cell adhesion, Fibroblast, Polystyrene, Protein adsorption, Quartz crystal microbalance, Tantalum, cell adhesion, quartz crystal microbalance, fibroblast, protein adsorption, polystyrene, tantalum, Animals, Cattle, Sheep, Mice, Fibroblasts, Serum, Cells, Cultured, NIH 3T3 Cells, Extracellular Matrix, Tantalum, Polystyrenes, Cycloheximide, Serum Albumin, Bovine, Fibronectins, Microscopy, Fluorescence, Protein Synthesis Inhibitors, Coated Materials, Biocompatible, Cell Culture Techniques, Substrate Specificity, Oxidation-Reduction, Cell Adhesion, Adsorption, Time Factors, Quartz, Adsorption, Animals, Cattle, Cell Adhesion, Cell Culture Techniques, Cells, Cultured, Coated Materials, Biocompatible, Cycloheximide, Extracellular Matrix, Fibroblasts, Fibronectins, Mice, Microscopy, Fluorescence, NIH 3T3 Cells, Oxidation-Reduction, Polystyrenes, Protein Synthesis Inhibitors, Quartz, Serum, Serum Albumin, Bovine, Sheep, Substrate Specificity, Tantalum, Time Factors, Cell adhesion, Quartz crystal microbalance, Fibroblast, Protein adsorption, Polystyrene, Tantalum, Biomedical Engineering
dc.subject Cell adhesion; Fibroblast; Polystyrene; Protein adsorption; Quartz crystal microbalance; Tantalum; cell adhesion; quartz crystal microbalance; fibroblast; protein adsorption; polystyrene; tantalum; Animals; Cattle; Sheep; Mice; Fibroblasts; Serum; Cells, Cultured; NIH 3T3 Cells; Extracellular Matrix; Tantalum; Polystyrenes; Cycloheximide; Serum Albumin, Bovine; Fibronectins; Microscopy, Fluorescence; Protein Synthesis Inhibitors; Coated Materials, Biocompatible; Cell Culture Techniques; Substrate Specificity; Oxidation-Reduction; Cell Adhesion; Adsorption; Time Factors; Quartz; Adsorption; Animals; Cattle; Cell Adhesion; Cell Culture Techniques; Cells, Cultured; Coated Materials, Biocompatible; Cycloheximide; Extracellular Matrix; Fibroblasts; Fibronectins; Mice; Microscopy, Fluorescence; NIH 3T3 Cells; Oxidation-Reduction; Polystyrenes; Protein Synthesis Inhibitors; Quartz; Serum; Serum Albumin, Bovine; Sheep; Substrate Specificity; Tantalum; Time Factors; Cell adhesion; Quartz crystal microbalance; Fibroblast; Protein adsorption; Polystyrene; Tantalum; Biomedical Engineering
dc.title Extracellular matrix remodelling during cell adhesion monitored by the quartz crystal microbalance
dc.type Journal Article
dc.parent Biomaterials
dc.journal.volume 17
dc.journal.volume 29
dc.journal.number 17 en_US
dc.publocation Oxford, UK en_US
dc.identifier.startpage 2581 en_US
dc.identifier.endpage 2587 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0903 Biomedical Engineering
dc.personcode 0000048911 en_US
dc.personcode 0000048927 en_US
dc.personcode 0000048929 en_US
dc.personcode 0000048928 en_US
dc.personcode 0000048915 en_US
dc.personcode 0000048931 en_US
dc.personcode 0000048930 en_US
dc.personcode 105631 en_US
dc.percentage 100 en_US
dc.classification.name Biomedical Engineering en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Cell adhesion; Quartz crystal microbalance; Fibroblast; Protein adsorption; Polystyrene; Tantalum en_US
dc.description.keywords Cell adhesion
dc.description.keywords Quartz crystal microbalance
dc.description.keywords Fibroblast
dc.description.keywords Protein adsorption
dc.description.keywords Polystyrene
dc.description.keywords Tantalum
dc.description.keywords Cell adhesion
dc.description.keywords Fibroblast
dc.description.keywords Polystyrene
dc.description.keywords Protein adsorption
dc.description.keywords Quartz crystal microbalance
dc.description.keywords Tantalum
dc.description.keywords Cell adhesion
dc.description.keywords Fibroblast
dc.description.keywords Polystyrene
dc.description.keywords Protein adsorption
dc.description.keywords Quartz crystal microbalance
dc.description.keywords Tantalum
dc.staffid 105631 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies


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