Lysozyme interaction with poly(HEMA)-based hydrogel

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dc.contributor.author Lord, MS
dc.contributor.author Stenzel, MH
dc.contributor.author Simmons, A
dc.contributor.author Milthorpe, BK
dc.date.accessioned 2010-07-15T07:27:21Z
dc.date.issued 2006-03
dc.identifier.citation Biomaterials, 2006, 27 (8), pp. 1341 - 1345
dc.identifier.issn 0142-9612
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/12813
dc.description.abstract Lysozyme interaction with an acrylic-based hydrogel, poly(2-hydroxyethyl methacrylate) co-methacrylic acid (P(HEMA-MAA)), was investigated using a combination of quartz crystal microbalance with dissipation (QCM-D), surface plasmon resonance (SPR) and dual polarisation interferometry (DPI). This combination of techniques demonstrated that lysozyme initially absorbed into the hydrogel matrix and displaced water from the hydrogel while subsequent lysozyme additions were adsorbed onto the surface of the hydrogel material. QCM-D, being sensitive to bound water, showed an overall decrease in mass and stiffening of the layer after lysozyme addition. SPR, a water insensitive technique, showed a net mass increase after addition of lysozyme and buffer rinses. DPI showed that the first exposure of lysozyme to P(HEMA-MAA) was consistent with lysozyme absorption while subsequent lysozyme exposures were consistent with lysozyme adsorption. © 2005 Elsevier Ltd. All rights reserved.
dc.language eng
dc.relation.isbasedon 10.1016/j.biomaterials.2005.09.007
dc.title Lysozyme interaction with poly(HEMA)-based hydrogel
dc.type Journal Article
dc.parent Biomaterials
dc.journal.volume 8
dc.journal.volume 27
dc.journal.number 8 en_US
dc.publocation Oxford, UK en_US
dc.identifier.startpage 1341 en_US
dc.identifier.endpage 1345 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0903 Biomedical Engineering
dc.personcode 105631
dc.percentage 100 en_US
dc.classification.name Biomedical Engineering en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Contact lens
dc.description.keywords Hydrogels
dc.description.keywords Lysozyme
dc.description.keywords Protein adsorption
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false


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