Gene expression profiling of HUH7-ins: lack of a granulogenic function for chromogranin A.

DSpace/Manakin Repository

Search OPUS


Advanced Search

Browse

My Account

Show simple item record

dc.contributor.author Lutherborrow, MA
dc.contributor.author Appavoo, M
dc.contributor.author Simpson, AM
dc.contributor.author Tuch, BE
dc.date.accessioned 2011-02-07T06:18:21Z
dc.date.issued 2009
dc.identifier.citation Islets, 2009, 1 (1), pp. 62 - 74
dc.identifier.issn 1938-2014
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13048
dc.description.abstract Previously, the insulin producing liver cell line HUH7-ins has been shown to synthesize, store and secrete insulin in response to glucose via secretory granules. The current study characterized the gene expression profile of HUH7-ins with the aim to identify changes possibly involved in the formation of granules. Additionally, experiments were conducted to determine the influence of chromogranin A (CgA) on secretory granule biogenesis (SGB) in HUH7-ins. Expression of 165 genes were significantly changed in HUH7-ins,though interestingly the majority of secretory granule associated genes, such as the chromogranins were unchanged. CgA was over-expressed in glucose unresponsive HUH7-ins cells to test whether CgA played a role in SGB and would restore the regulated secretory phenotype. Over-expression affected neither the storage nor regulated secretion of insulin. These data suggest that SGB may by regulated at a post-transcriptional level with no evidence to indicate that CgA regulates SGB in the cell line HUH7-ins.
dc.format Print
dc.language eng
dc.relation.isbasedon 10.4161/isl.1.1.8994
dc.title Gene expression profiling of HUH7-ins: lack of a granulogenic function for chromogranin A.
dc.type Journal Article
dc.parent Islets
dc.journal.volume 1
dc.journal.volume 1
dc.journal.number 1 en_US
dc.publocation USA en_US
dc.identifier.startpage 60 en_US
dc.identifier.endpage 72 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 1108 Medical Microbiology
dc.personcode 940084
dc.percentage 100 en_US
dc.classification.name Medical Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Humans
dc.description.keywords Secretory Vesicles
dc.description.keywords Insulin
dc.description.keywords Glucose
dc.description.keywords Microarray Analysis
dc.description.keywords Gene Expression Profiling
dc.description.keywords Gene Expression Regulation
dc.description.keywords Cell Line, Tumor
dc.description.keywords Insulin-Secreting Cells
dc.description.keywords Chromogranin A
dc.description.keywords Middle Aged
dc.description.keywords Validation Studies as Topic
dc.description.keywords Male
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)


Files in this item

This item appears in the following Collection(s)

Show simple item record