Failure of the biotic ligand and free-ion activity models to explain zinc bioaccumulation by Chlorella kesslerii

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dc.contributor.author Hassler, CS
dc.contributor.author Wilkinson, KJ
dc.date.accessioned 2011-02-07T06:18:38Z
dc.date.issued 2003-01
dc.identifier.citation Environmental Toxicology And Chemistry, 2003, 22 (3), pp. 620 - 626
dc.identifier.issn 0730-7268
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13080
dc.description.abstract Zinc accumulation by Chlorella kesslerii (Chlorophycee) was studied for [Zn2+] ranging from 4 pM to I mM. A first-order uptake flux as predicted by the free ion activity model (FIAM) and the biotic ligand model (BLM) was not observed. Furthermore, when algae were preconditioned in slightly limiting (10 pM) versus optimal (I nM) free zinc concentrations, the internalization flux increased and was nearly constant over the range of [Zn2+] examined. This observation was attributed to the synthesis of membrane-bound zinc transporters. Neither solution Zn chemistry nor surface-bound Zn was a good predictor of Zn uptake fluxes. Several hypotheses were examined to explain the failure of the steady-state uptake models. Although zinc excretion and a Zn diffusion limitation were observed, neither result could explain the majority of observed deviation from the models. Bioaccumulation experiments performed with inhibitors (vanadate and carbonyl cyanide m-chlorophenylhydrazone [CCCP]) demonstrated clearly that zinc transport is an energy-dependent process in Chlorella kesslerii. The presence of an active transport signifies that Zn uptake may function independently of the electrochemical Zn gradient and that, in some cases, both uptake fluxes and receptor-bound Zn may be independent of solution chemistry.
dc.format No Code
dc.publisher Setac
dc.relation.isbasedon 10.1897/1551-5028(2003)022<0620:FOTBLA>2.0.CO;2
dc.subject zinc, Chlorella, free ion activity model, biotic ligand model, uptake, Environmental Sciences
dc.subject zinc; Chlorella; free ion activity model; biotic ligand model; uptake; Environmental Sciences
dc.title Failure of the biotic ligand and free-ion activity models to explain zinc bioaccumulation by Chlorella kesslerii
dc.type Journal Article
dc.parent Environmental Toxicology And Chemistry
dc.journal.volume 3
dc.journal.volume 22
dc.journal.number 3 en_US
dc.publocation Pensacola, USA en_US
dc.identifier.startpage 620 en_US
dc.identifier.endpage 626 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0399 Other Chemical Sciences
dc.personcode 107808 en_US
dc.personcode 0000054117 en_US
dc.percentage 100 en_US
dc.classification.name Other Chemical Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords zinc; Chlorella; free ion activity model; biotic ligand model; uptake en_US
dc.description.keywords zinc
dc.description.keywords zinc
dc.description.keywords zinc
dc.description.keywords Chlorella
dc.description.keywords Chlorella
dc.description.keywords Chlorella
dc.description.keywords Chlorella
dc.description.keywords free ion activity model
dc.description.keywords free ion activity model
dc.description.keywords free ion activity model
dc.description.keywords free ion activity model
dc.description.keywords biotic ligand model
dc.description.keywords biotic ligand model
dc.description.keywords biotic ligand model
dc.description.keywords biotic ligand model
dc.description.keywords uptake
dc.description.keywords uptake
dc.description.keywords uptake
dc.description.keywords uptake
dc.description.keywords zinc
dc.description.keywords zinc
dc.description.keywords Chlorella
dc.description.keywords Chlorella
dc.description.keywords free ion activity model
dc.description.keywords free ion activity model
dc.description.keywords biotic ligand model
dc.description.keywords biotic ligand model
dc.description.keywords uptake
dc.description.keywords uptake
dc.staffid en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science


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