Molecular-Cloning And Characterization Of Protective Outer-Membrane Protein-P.69 From Bordetella-Pertussis

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dc.contributor.author Charles, IG
dc.contributor.author Dougan, G
dc.contributor.author Pickard, D
dc.contributor.author Chatfield, S
dc.contributor.author Smith, MA
dc.contributor.author Novotny, P
dc.contributor.author Morrissey, PE
dc.contributor.author Fairweather, N
dc.date.accessioned 2011-02-07T06:20:02Z
dc.date.issued 1989-01
dc.identifier.citation Proceedings Of The National Academy Of Sciences Of The United States Of America, 1989, 86 (10), pp. 3554 - 3558
dc.identifier.issn 0027-8424
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13236
dc.description.abstract Protein P.69 is localized on the outer membrane of Bordetella pertussis and is one of the virulence factors believed to contribute to the disease state of whooping cough. We demonstrate that protein synthesis of P.69 is under genetic control of the vir locus. Using oligonucleotide probes derived from the protein sequence of a cyanogen bromide fragment, we have cloned the gene for P.69 from B. pertussis CN2992. Analysis of the DNA sequence reveals a G + C-rich gene capable of encoding a protein of 910 amino acids with a Mr of 93,478, suggesting that P.69 is a processed form of a larger precursor. In common with some of the genes in the pertussis toxin operon, the sequence CCTGG was found 5' to the ATG initiation codon. At the 3' end, 29 bases after the TAA stop codon, the sequence GTTTTTCCT was found and may have some function in transcription termination. A full-length clone of the gene for P.69 carried by the cosmid pBPI69 was unable to direct the expression of P.69 protein in an Escherichia coli host. The generation of P.69-fusion products allowed the detection of P.69-specific protein products synthesized in E. coli.
dc.format Yes
dc.publisher Natl Acad Sciences
dc.title Molecular-Cloning And Characterization Of Protective Outer-Membrane Protein-P.69 From Bordetella-Pertussis
dc.type Journal Article
dc.parent Proceedings Of The National Academy Of Sciences Of The United States Of America
dc.journal.volume 10
dc.journal.volume 86
dc.journal.number 10 en_US
dc.publocation Washington en_US
dc.identifier.startpage 3554 en_US
dc.identifier.endpage 3558 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 060409 Molecular Evolution
dc.personcode 100304
dc.personcode 109028
dc.percentage 100 en_US
dc.classification.name Molecular Evolution en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:A1989U652300025 en_US
dc.location.activity ISI:A1989U652300025
dc.description.keywords NA
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Elec, Mech and Mechatronic Systems
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)


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