Three-dimensional elemental bio-imaging of Fe, Zn, Cu, Mn and P in a 6-hydroxydopamine lesioned mouse brain

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dc.contributor.author Hare, DJ
dc.contributor.author George, JL
dc.contributor.author Grimm, R
dc.contributor.author Wilkins, S
dc.contributor.author Adlard, PA
dc.contributor.author Cherny, RA
dc.contributor.author Bush, AI
dc.contributor.author Finkelstein, DI
dc.contributor.author Doble, P
dc.date.accessioned 2011-02-07T06:20:27Z
dc.date.issued 2010-11
dc.identifier.citation Metallomics, 2010, 2 (11), pp. 745 - 753
dc.identifier.issn 1756-5901
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/13287
dc.description.abstract Three dimensional maps of iron (Fe), zinc (Zn), copper (Cu), manganese (Mn) and phosphorous (P) in a 6-hydroxydopamine (6-OHDA) lesioned mouse brain were constructed employing a novel quantitative laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) imaging method known as elemental bio-imaging. The 3D maps were produced by ablating serial consecutive sections taken from the same animal. Each section was quantified against tissue standards resulting in a three dimensional map that represents the variation of trace element concentrations of the mouse brain in the area surrounding the substantia nigra (SN). Damage caused by the needle or the toxin did not alter the distribution of Zn, and Cu but significantly altered Fe in and around the SN and both Mn and Fe around the needle track. A 20% increase in nigral Fe concentration was observed within the lesioned hemisphere. This technique clearly shows the natural heterogeneous distributions of these elements throughout the brain and the perturbations that occur following trauma or intoxication. The method may applied to three-dimensional modelling of trace elements in a wide range of tissue samples. © 2010 The Royal Society of Chemistry.
dc.language eng
dc.relation.isbasedon 10.1039/c0mt00039f
dc.title Three-dimensional elemental bio-imaging of Fe, Zn, Cu, Mn and P in a 6-hydroxydopamine lesioned mouse brain
dc.type Journal Article
dc.description.version Published
dc.parent Metallomics
dc.journal.volume 11
dc.journal.volume 2
dc.journal.number 11 en_US
dc.publocation Cambridge en_US
dc.identifier.startpage 745 en_US
dc.identifier.endpage 753 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0601 Biochemistry and Cell Biology
dc.personcode 010494
dc.personcode 995243
dc.percentage 100 en_US
dc.classification.name Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:000283743700003 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Forensic Science
utslib.copyright.status Open Access
utslib.copyright.date 2015-04-15 12:23:47.074767+10
pubs.consider-herdc true
utslib.collection.history General (ID: 2)
utslib.collection.history School of Chemistry and Forensic Science (ID: 339)
utslib.collection.history School of Chemistry and Forensic Science (ID: 339)


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