Quantifying microbial diversity: Morphotypes, 16s rRNA genes, and carotenoids of oxygenic phototrophs in microbial mats

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dc.contributor.author Nübel, U
dc.contributor.author Garcia-Pichel, F
dc.contributor.author Kühl, M
dc.contributor.author Muyzer, G
dc.date.accessioned 2011-02-07T06:20:28Z
dc.date.issued 1999-02
dc.identifier.citation Applied and Environmental Microbiology, 1999, 65 (2), pp. 422 - 430
dc.identifier.issn 0099-2240
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13290
dc.description.abstract We quantified the diversity of oxygenic phototrophic microorganisms present in eight hypersaline microbial mats on the basis of three cultivation-independent approaches. Morphological diversity was studied by microscopy. The diversity of carotenoids was examined by extraction from mat samples and high-pressure liquid chromatography analysis. The diversity of 16S rRNA genes from oxygenic phototrophic microorganisms was investigated by extraction of total DNA from mat samples, amplification of 16S rRNA gene segments from cyanobacteria and plastids of eukaryotic algae by phylum- specific PCR, and sequence-dependent separation of amplification products by denaturing-gradient gel electrophoresis. A numerical approach was introduced to correct for crowding the results of chromatographic and electrophoretic analyses. Diversity estimates typically varied up to twofold among mats. The congruence of richness estimates and Shannon-Weaver indices based on numbers and proportional abundances of unique morphotypes, 16S rRNA genes, and carotenoids unveiled the underlying diversity of oxygenic phototrophic microorganisms in the eight mat communities studied.
dc.language eng
dc.title Quantifying microbial diversity: Morphotypes, 16s rRNA genes, and carotenoids of oxygenic phototrophs in microbial mats
dc.type Journal Article
dc.description.version Published
dc.parent Applied and Environmental Microbiology
dc.journal.volume 2
dc.journal.volume 65
dc.journal.number 2 en_US
dc.publocation Washington DC, USA en_US
dc.identifier.startpage 422 en_US
dc.identifier.endpage 430 en_US
dc.cauo.name SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 0602 Ecology
dc.for 0605 Microbiology
dc.personcode 107129
dc.percentage 50 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - C3


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