The commercially available restriction enzyme BspHl is blocked by overlapping methylation

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dc.contributor.author Servos, S
dc.contributor.author Silva, C
dc.contributor.author Dougan, G
dc.contributor.author Charles, IG
dc.date.accessioned 2011-02-07T06:20:52Z
dc.date.issued 1991-01
dc.identifier.citation Nucleic Acids Research, 1991, 19 (1), pp. 183 - 183
dc.identifier.issn 0305-1048
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13341
dc.description.abstract The restriction endonuclease Bspill. conunercially available from New England Biolabs (Bishop's Stortford, UK), cleaves the site T/CATGA. This restriction enzyme is of particular use in the construction of gene expression cassettes in the vector pKK233 -2 (I) as it is one of the three enzymes along with AflIII and NcoI that has an 'ATG' in its recognition sequence. In this paper we show that BspHI is Dam methylase (2) sensitive and will not cleave DNA that has the overlapping sequ~nce GATC. The oligonucleotides: OS3142 5' -CTAGATCATGAGCGAACTG~ TCGTGAACGTGATCAAAGCTTGGTAC- 3' and OS3143 5'-CAAGCTTTGATCACGTTCACGATCAGTTCGCTCATGAT- 3' were annealed and ligated into XbaI-KpnI digested bluescript SKI1+ (Stratagene, Cambridge, UK}.
dc.format Yes
dc.publisher Oxford Univ Press United Kingdom
dc.relation.isbasedon 10.1093/nar/19.1.183
dc.title The commercially available restriction enzyme BspHl is blocked by overlapping methylation
dc.type Journal Article
dc.parent Nucleic Acids Research
dc.journal.volume 1
dc.journal.volume 19
dc.journal.number 1 en_US
dc.publocation Oxford en_US
dc.identifier.startpage 183 en_US
dc.identifier.endpage 183 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 1101 Medical Biochemistry and Metabolomics
dc.for 0604 Genetics
dc.for 0601 Biochemistry and Cell Biology
dc.personcode 109028
dc.percentage 34 en_US
dc.classification.name Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)


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