Isolation And Computer-Aided Characterization Of Mmei, A Type-Ii Restriction Endonuclease From Methylophilus-Methylotrophus

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dc.contributor.author Boyd, AJ
dc.contributor.author Charles, IG
dc.contributor.author Keyte, J
dc.contributor.author Brammar, W
dc.date.accessioned 2011-02-07T06:21:13Z
dc.date.issued 1986-01
dc.identifier.citation Nucleic Acids Research, 1986, 14 (13), pp. 5255 - 5274
dc.identifier.issn 0305-1048
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13386
dc.description.abstract A Type II restriction endonuclease, MmeI, has been purified from the obligate methylotroph, Methylophilus methylotrophus. The enzyme was shown to have the non-palindromic recognition sequence 5'-T C C Pu A C (N)20-3', 3'-A G G Py T G (N)18-5' and to cleave (as indicated) on the 3' side, generating a two nucleotide 3' projection. Determination of the recognition sequence was achieved using two new computer programs; RECOG, which predicts recognition sequences from the pattern of restriction fragments obtained from DNAs of known sequence, and GELSIM, which generates graphical simulations of DNA band patterns obtained by gel electrophoresis of restriction digests of sequenced DNA molecules.
dc.format Yes
dc.publisher Oxford Univ Press United Kingdom
dc.relation.isbasedon 10.1093/nar/14.13.5255
dc.subject NA, Developmental Biology
dc.subject NA; Developmental Biology
dc.title Isolation And Computer-Aided Characterization Of Mmei, A Type-Ii Restriction Endonuclease From Methylophilus-Methylotrophus
dc.type Journal Article
dc.parent Nucleic Acids Research
dc.journal.volume 13
dc.journal.volume 14
dc.journal.number 13 en_US
dc.publocation Oxford en_US
dc.identifier.startpage 5255 en_US
dc.identifier.endpage 5274 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0601 Biochemistry and Cell Biology
dc.personcode 940308 en_US
dc.personcode 109028 en_US
dc.personcode 0000060129 en_US
dc.personcode 0000060130 en_US
dc.percentage 100 en_US
dc.classification.name Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:A1986D269500010 en_US
dc.location.activity ISI:A1986D269500010
dc.description.keywords NA en_US
dc.description.keywords NA
dc.staffid en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/DVC (Corporate Services)
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3


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