Molecular cloning and characterization of the aroD gene encoding 3-dehydroquinase from Salmonella typhi

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Show simple item record Servos, S Chatfield, S Hone, D Levine, M Dimitriadis, G Pickard, D Dougan, G Fairweather, N Charles, IG 2011-02-07T06:21:18Z 1991-01
dc.identifier.citation Journal Of General Microbiology, 1991, 137 (1), pp. 147 - 152
dc.identifier.issn 0022-1287
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract The aroD gene from Salmonella typhi, encoding 5-dehydroquinate hydrolyase (3-dehydroquinase), has been cloned into Escherichia coli and the DNA sequence determined. The aroD gene was isolated from a cosmid gene bank by complementation of an S. typhimurium aroD mutant. Analysis of the DNA sequence revealed the presence of an open reading frame capable of encoding a protein of 252 amino acids with a calculated Mr of 27706. Comparison of the deduced S. typhi 3-dehydroquinase protein sequence with that elucidated for E. coli revealed 69% homology. Alignment of the S. typhi sequence and equivalent Aspergillus nidulans and Saccharomyces cerevisiae sequences showed that homology was lower, at 24%, but still significant. Use of a minicell expression system demonstrated that a polyclonal antibody raised against E. coli 3-dehydroquinase cross-reacted with its S. typhi counterpart.
dc.format Yes
dc.publisher Soc General Microbiology
dc.title Molecular cloning and characterization of the aroD gene encoding 3-dehydroquinase from Salmonella typhi
dc.type Journal Article
dc.parent Journal Of General Microbiology
dc.journal.volume 1
dc.journal.volume 137
dc.journal.number 1 en_US
dc.publocation Reading en_US
dc.identifier.startpage 147 en_US
dc.identifier.endpage 152 en_US SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 109028
dc.percentage 100 en_US Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords NA
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)
utslib.collection.history Uncategorised (ID: 363)

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