Cloning and Characterization of the aroA Gene from Mycobacterium tuberculosis

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dc.contributor.author Garbe, T
dc.contributor.author Jones, C
dc.contributor.author Charles, IG
dc.contributor.author Dougan, G
dc.contributor.author Young, D
dc.date.accessioned 2011-02-07T06:24:11Z
dc.date.issued 1990-01
dc.identifier.citation Journal Of Bacteriology, 1990, 172 (12), pp. 6774 - 6782
dc.identifier.issn 0021-9193
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/13720
dc.description.abstract The aroA gene from Mycobacterium tuberculosis has been cloned by complementation of an aroA mutant of Escherichia coli after lysogenization with a recombinant DNA library in the lambda gt11 vector. Detailed characterization of the M. tuberculosis aroA gene by nucleotide sequencing and by immunochemical analysis of the expressed product indicates that it encodes a 5-enolpyruvylshikimate-3-phosphate synthase that is structurally related to analogous enzymes from other bacterial, fungal, and plant sources. The potential use of the cloned gene in construction of genetically defined mutant strains of M. tuberculosis by gene replacement is proposed as a novel approach to the rational attenuation of mycobacterial pathogens and the possible development of new antimycobacterial vaccines.
dc.format Yes
dc.publisher Amer Soc Microbiology
dc.title Cloning and Characterization of the aroA Gene from Mycobacterium tuberculosis
dc.type Journal Article
dc.parent Journal Of Bacteriology
dc.journal.volume 12
dc.journal.volume 172
dc.journal.number 12 en_US
dc.publocation Washington DC, USA en_US
dc.publocation Auckland, New Zealand
dc.identifier.startpage 6774 en_US
dc.identifier.endpage 6782 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.conference Pacific Rim Real Estate Conference
dc.for 1108 Medical Microbiology
dc.personcode 109028
dc.percentage 100 en_US
dc.classification.name Medical Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.date.activity 2006-01-22
dc.location.activity en_US
dc.location.activity Auckland, New Zealand
dc.description.keywords NA
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)


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