Gamma irradiation as a biological decontaminant and its effect on common fingermark detection techniques and DNA profiling

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dc.contributor.author Hoile, RJ
dc.contributor.author Banos, C
dc.contributor.author Colella, M
dc.contributor.author Walsh, SJ
dc.contributor.author Roux, CP
dc.date.accessioned 2011-02-07T06:24:44Z
dc.date.issued 2010-01
dc.identifier.citation Journal of Forensic Sciences, 2010, 55 (1), pp. 171 - 177
dc.identifier.issn 0022-1198
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/13788
dc.description.abstract The use of disease-causing organisms and their toxins against the civilian population has defined bioterrorism and opened forensic science up to the challenges of processing contaminated evidence. This study sought to detennine the use of gamma irradiation as an effective biological decontaminant and its effect on the recovery of latent fingennarks from both porous and nonporous items. Test items were contaminated with viable spores marked with latent pIinrs and then decontaminated using a cobalt 60 gamma irradiator. Fingerrnark detection was the focus with standard methods inclurJing 1,2-inrJanedione, ninhydrin, diazafluoren-9-one, and physical developer used during this study. DNA recovery using 20% Chelex extraction and quantitative real-time polymerdse chain reaction was also explored. Gamma irradiation proved effective as a bacterial decontaminant with D-values ranging from 458 to 500 Gy for nonporous items and 797-808 Gy for porous ones. The results demonstrated the successful recovel)' of latent marks and DNA establishing gamma irradiation as a viable decontamination option.
dc.publisher Blackwell Publishing Ltd
dc.relation.isbasedon 10.1111/j.1556-4029.2009.01233.x
dc.title Gamma irradiation as a biological decontaminant and its effect on common fingermark detection techniques and DNA profiling
dc.type Journal Article
dc.parent Journal of Forensic Sciences
dc.journal.volume 1
dc.journal.volume 55
dc.journal.number en_US
dc.journal.number 1 en_US
dc.publocation USA en_US
dc.identifier.startpage 171 en_US
dc.identifier.endpage 177 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0399 Other Chemical Sciences
dc.personcode 02103822
dc.personcode 960382
dc.personcode 109664
dc.percentage 100 en_US
dc.classification.name Other Chemical Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords forensic science, gamma irradiation, microbial forensics, latent fingennarks, DNA, bioterrorism
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Forensic Science
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history Closed (ID: 3)
utslib.collection.history School of Chemistry and Forensic Science (ID: 339)


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