Resistance To Chloramphenicol In Proteus-Mirabilis By Expression Of A Chromosomal Gene For Chloramphenicol Acetyltransferase

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Show simple item record Charles, IG Harford, S Brookfield, J Shaw, W 2011-02-07T06:24:48Z 1985-01
dc.identifier.citation Journal Of Bacteriology, 1985, 164 (1), pp. 114 - 122
dc.identifier.issn 0021-9193
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract Proteus mirabilis PM13 is a well-characterized chloramphenicol-sensitive isolate which spontaneously gives rise to resistant colonies on solid media containing chloramphenicol (50 micrograms ml-1) at a plating efficiency of 10(-4) to 10(-5). Such chloramphenicol-resistant colonies exhibit a novel phenotype with respect to chloramphenicol resistance. When a single colony grown on chloramphenicol agar is transferred to liquid medium and grown in the absence of antibiotic for 150 generations, a population of predominantly sensitive cells arises. This mutation-reversion phenomenon has been observed in other Proteus species and Providencia strains, wherein resistance has been shown to be mediated in each case by the enzyme chloramphenicol acetyltransferase. The cat gene responsible for the phenomenon is chromosomal and can be cloned from P. mirabilis PM13 with DNA prepared from cells grown in the absence or the presence of chloramphenicol. Recombinant plasmids which confer resistance to chloramphenicol carry an 8.5-kilobase PstI fragment irrespective of the source of host DNA. The location of the cat gene within the PstI fragment was determined by Southern blotting with a cat consensus oligonucleotide corresponding to the expected amino acid sequence of the active site region of chloramphenicol acetyltransferase, and the direction of transcription was deduced from homology with the type I cat variant.
dc.format Yes
dc.publisher Amer Soc Microbiology
dc.title Resistance To Chloramphenicol In Proteus-Mirabilis By Expression Of A Chromosomal Gene For Chloramphenicol Acetyltransferase
dc.type Journal Article
dc.parent Journal Of Bacteriology
dc.journal.volume 1
dc.journal.volume 164
dc.journal.number 1 en_US
dc.publocation Washington en_US
dc.identifier.startpage 114 en_US
dc.identifier.endpage 122 en_US SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 1108 Medical Microbiology
dc.personcode 109028
dc.percentage 100 en_US Medical Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity ISI:A1985ASA7400017 en_US
dc.location.activity ISI:A1985ASA7400017
dc.location.activity ISI:A1985ASA7400017
dc.description.keywords NA en_US
dc.description.keywords NA
dc.description.keywords NA
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10

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