Development of a mild mercaptoethanol extraction method for determination of mercury species in biological samples by HPLC-ICP-MS

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Show simple item record Wang, M Feng, W Shi, J Zhang, F Wang, B Zhu, M Li, B Zhao, Y Chai, Z 2012-02-02T05:06:02Z 2007-01
dc.identifier.citation Talanta, 2007, 71 (5), pp. 2034 - 2039
dc.identifier.issn 0039-9140
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract A mild, efficient and convenient extraction method of using 2-mercaptoethanol contained extractant solution combined with an incubator shaker for determination of mercury species in biological samples by HPLCICP-MS has been developed. The effects of the concentration of 2-mercaptoethanol, the composition of the extractant solution and the shaking time on the efficiency of mercury extraction were evaluated. The optimization experiments indicated that the quantitative extraction of mercury species from biological samples could be achieved by using 0.1% (v/v) HCl, 0.1% (v/v) 2-mercapoethanol and 0.15% (m/v) KCl extractant solution in an incubator shaker for shaking overnight (about 12 h) at room temperature. The established method was validated by analysis of various biological certified reference materials, including NRCC DOLT-3 (dogfish liver), IAEA 436 (tuna fish), IAEA MA-B-3/TM (garfish filet), IAEA MA-M-2/TM (mussel tissue), GBW 08193 (bovine liver) and GBW 08572 (prawn). The analytical results of the reference materials were in good agreement with the certified or reference values of both methyl and total mercury, indicating that no distinguishable transformation between mercury species had occurred during the extraction and determination procedures. The limit of detection (LOD) for methyl (CH3Hg+) and inorganic mercury (Hg2+) by the method are both as 0.2 gL-1. The relative standard deviation (R.S.D.s) for CH3Hg+ and Hg2+ are 3.0% and 5.8%, respectively. The advantages of the developed extraction method are that (1) it is easy to operate in HPLCICP-MS for mercury species determination since the extracted solution can be directly injected into the HPLC column without pH adjustment and (2) the memory effect of mercury in the ICP-MS measurement system can be reduced.
dc.publisher Elsevier Inc
dc.relation.isbasedon 10.1016/j.talanta.2006.09.012
dc.title Development of a mild mercaptoethanol extraction method for determination of mercury species in biological samples by HPLC-ICP-MS
dc.type Journal Article
dc.parent Talanta
dc.journal.volume 5
dc.journal.volume 71
dc.journal.number 5 en_US
dc.publocation Amsterdam, Netherlands en_US
dc.identifier.startpage 2034 en_US
dc.identifier.endpage 2039 en_US SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0301 Analytical Chemistry
dc.personcode 112014
dc.percentage 100 en_US Analytical Chemistry en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords Mercury speciation; Mild extraction; HPLC?ICP-MS; Biological sample; Memory effect en_US
dc.description.keywords Mercury speciation
dc.description.keywords Mild extraction
dc.description.keywords HPLCICP-MS
dc.description.keywords Biological sample
dc.description.keywords Memory effect
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
utslib.collection.history Uncategorised (ID: 363)
utslib.collection.history Closed (ID: 3)

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