Lab-on-a-chip biosensor for glucose based on a packed immobilized enzyme reactor

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Show simple item record Blanes, L Mora, MF do Lago, CL Ayon, A Garcia, CD 2012-02-02T05:33:02Z 2007-01
dc.identifier.citation Electroanalysis, 2007, 19 (23), pp. 2451 - 2456
dc.identifier.issn 1040-0397
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract In this work, the development of a packed immobilized enzyme reactor (IMER) and its integration to a capillary electrophoresis microchip is described. The present microchip design differs from others, in the fact that the same design could be used with or without the particles and, just by changing the material used to pack the IMER, different analytes can be detected. The applied procedure involves the separation of the target analyte by capillary electrophoresis (CE), which is then coupled to a post-column IMER that produces H2O2. The H2O2 produced is finally detected downstream at the surface of a working electrode. Glucose was detected above 100 mM by packing particles modified with glucose oxidase at the end of the separation channel. The analytical performance of the microchip-CE has been demonstrated by performing the separation and detection of glucose and noradrenaline. Additions of fructose showed no effect on either the peak position or the peak magnitude of glucose. The microchip-CE-IMER was also used to quantify glucose in carbonated beverages with good agreement with other reports.
dc.publisher Wiley - VCH Verlag GmbH & Co. KGaA
dc.relation.isbasedon 10.1002/elan.200704001
dc.title Lab-on-a-chip biosensor for glucose based on a packed immobilized enzyme reactor
dc.type Journal Article
dc.parent Electroanalysis
dc.journal.volume 23
dc.journal.volume 19
dc.journal.number 23 en_US
dc.publocation Germany en_US
dc.publocation New York, USA
dc.identifier.startpage 2451 en_US
dc.identifier.endpage 2456 en_US SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.conference Academy of Management Conference
dc.for 0399 Other Chemical Sciences
dc.personcode 105015
dc.percentage 100 en_US Other Chemical Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US 2011-08-12
dc.location.activity en_US
dc.location.activity San Antonio, USA
dc.description.keywords Capillary electrophoresis, Microchips, Glucose, Immobilized enzyme reactor
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Forensic Science
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Uncategorised (ID: 363)
utslib.collection.history Closed (ID: 3)

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