Analysis of mercury-containing protein fractions in brain cytosol of the maternal and infant rats after exposure to a low-dose of methylmercury by SEC coupled to isotope dilution ICP-MS

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dc.contributor.author Wang, M
dc.contributor.author Feng, W
dc.contributor.author Wang, H
dc.contributor.author Zhang, Y
dc.contributor.author Li, J
dc.contributor.author Li, B
dc.contributor.author Zhao, Y
dc.contributor.author Chai, Z
dc.date.accessioned 2012-02-02T05:34:03Z
dc.date.issued 2008-01
dc.identifier.citation Journal of Analytical Atomic Spectrometry, 2008, 23 (8), pp. 1112 - 1116
dc.identifier.issn 0267-9477
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/14659
dc.description.abstract The mercury-containing protein fractions in the brain cytosol of the maternal and infant rats after exposure to low-dose of methylmercury chloride (0.3 mg Hg kg1 day1) were analyzed by a SEC-ICPMS method with the postcolumn isotope dilution analysis. The enriched spiking including 34S, 65Cu, 67Zn and 198Hg was continuously added into the eluate from the HPLC column and then the isotopediluted fractions were on-line measured by ICP-MS. Therefore, the absolute amounts of sulfur, mercury, zinc and copper in the eluted protein fractions could be attained after calculation of the corresponding peak areas in the mass flow chromatogram. Five mercury-containing protein fractions were monitored in the maternal sample, whereas only one was in the infant sample. The mercury content in the infant sample was about 10 times lower than the one in the maternal sample. In the meantime, two copper- and four zinc-containing fractions were found in both maternal and infant samples. The detection limit for S, Cu, Zn and Hg are 11, 0.1, 1.5 and 0.2 ng, respectively. The results demonstrate that different mercury-containing protein fractions may exist in brain cytosol between maternal and infant rats and the quantitative calculation may be helpful for the toxicological study.
dc.publisher Royal Society of Chemistry
dc.relation.isbasedon 10.1039/b802124d
dc.title Analysis of mercury-containing protein fractions in brain cytosol of the maternal and infant rats after exposure to a low-dose of methylmercury by SEC coupled to isotope dilution ICP-MS
dc.type Journal Article
dc.parent Journal of Analytical Atomic Spectrometry
dc.journal.volume 8
dc.journal.volume 23
dc.journal.number 8 en_US
dc.publocation Cambridge, UK en_US
dc.identifier.startpage 1112 en_US
dc.identifier.endpage 1116 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0301 Analytical Chemistry
dc.personcode 112014
dc.percentage 100 en_US
dc.classification.name Analytical Chemistry en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.location.activity ISI:A1996UL29000007
dc.description.keywords NA en_US
dc.description.keywords complete characterization
dc.description.keywords global optimality
dc.description.keywords generalized Farkas lemma
dc.description.keywords epsilon-subdifferential
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Open Access
utslib.copyright.date 2015-04-15 12:23:47.074767+10
utslib.collection.history General (ID: 2)


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