Aerotaxis in Desulfovibrio

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dc.contributor.author Eschemann, A
dc.contributor.author Kühl, M
dc.contributor.author Cypionka, H
dc.date.accessioned 2012-02-02T06:57:50Z
dc.date.issued 1999
dc.identifier.citation Environmental Microbiology, 1999, 1 (6), pp. 489 - 494
dc.identifier.issn 1462-2912
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/14823
dc.description.abstract Aerotaxis of two sulphate-reducing bacteria, the freshwater strain Desulfovibrio desulfuricans CSN (DSM 9104) and the marine strain Desulfovibrio oxyclinae N13 (DSM 11498), was studied using capillary microslides, microscopy and oxygen microsensors. The bacteria formed ring-shaped bands in oxygen diffusion gradients surrounding O2 bubbles, which were placed into anoxic sulphate-free cell suspensions in capillary microslides. The radial expansion of the oxic volume by diffusion was stopped by aerobic respiration. Bands were formed by cells avoiding high O2 levels near the O2 bubble, as well as by cells entering from the surrounding anoxic zone. At the inner edge of the bands, O2 levels of up to 20% air saturation (50μM O2) were found, while the outer edge always coincided with the oxic-anoxic interface. Ring diameters and O2 concentrations at the inner edge of the band depended on the cell density and the strain used in the suspension. Band formation did not occur in the absence of an electron donor (S mM lactate) or when N2 gas bubbles were used. Both strains were highly motile with velocities of ≈ 32 μm s-1 during forward runs, and 7 μm s-1 during backward runs respectively. Within the bands, cells moved in circles of about 20 μm diameter, while cells outside the band exhibited straighter or only slightly bent traces. It is concluded that the capacity of respiration at high rates and the positive and negative aerotactical responses of Desulfovibrio provide an efficient strategy for removing O2 from the habitat in situations where sufficient electron donors and high cell densities are present.
dc.language eng
dc.title Aerotaxis in Desulfovibrio
dc.type Journal Article
dc.description.version Published
dc.parent Environmental Microbiology
dc.journal.volume 6
dc.journal.volume 1
dc.journal.number 6 en_US
dc.publocation United Kingdom en_US
dc.identifier.startpage 489 en_US
dc.identifier.endpage 494 en_US
dc.cauo.name SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 107129
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - C3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)


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