Optical microsensors for analysis of microbial communities

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dc.contributor.author Kühl, M
dc.date.accessioned 2012-02-02T07:14:08Z
dc.date.issued 2005
dc.identifier.citation Methods in Enzymology, 2005, 397 pp. 166 - 199
dc.identifier.issn 0076-6879
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/14855
dc.description.abstract Fiber-optic microprobes connected to sensitive light meters are ideal tools to resolve the steep gradients of light intensity and spectral composition that prevail in aggregates and surface-associated microbial communities in sediments, biofilms, and microbial mats. They allow for a detailed mapping of light fields and enable insights to the complex optical properties of such highly light-scattering and -absorbing microbial systems. Used in combination with microsensors for chemical species, fiber-optic irradiance microprobes allow for detailed studies of photosynthesis regulation and of the photobiology of microbial phototrophs in intact samples under ambient microenvironmental conditions of the natural habitat. Fiber-optic microprobes connected to sensitive fluorometers enable microscale fluorescence measurements, which can be used to map (i) diffusivity and flow; (ii) distribution of photosynthetic microbes, via their photopigment autofluorescence; and (iii) activity of oxygenic photosynthesis via variable chlorophyll fluorescence measurements. Furthermore, by immobilizing optical indicator dyes on the end of optical fibers, fiber-optic microsensors for temperature, salinity, and chemical species such as oxygen, pH, and CO2 can be realized. Copyright 2005, Elsevier Inc. All rights reserved.
dc.language eng
dc.relation.isbasedon 10.1016/S0076-6879(05)97010-9
dc.title Optical microsensors for analysis of microbial communities
dc.type Journal Article
dc.description.version Published
dc.parent Methods in Enzymology
dc.journal.volume 397
dc.journal.number en_US
dc.publocation USA en_US
dc.publocation Australia
dc.identifier.startpage 166 en_US
dc.identifier.endpage 199 en_US
dc.cauo.name SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.conference 15th National Conference of Australian Society for Operations Research
dc.for 0601 Biochemistry and Cell Biology
dc.personcode 107129
dc.percentage 100 en_US
dc.classification.name Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.date.activity 1999-01-01
dc.location.activity en_US
dc.location.activity Gold Coast
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - C3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)
utslib.collection.history Uncategorised (ID: 363)


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