Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging

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dc.contributor.author Trampe, E
dc.contributor.author Kolbowski, J
dc.contributor.author Schreiber, U
dc.contributor.author Kühl, M
dc.date.accessioned 2012-02-02T07:42:40Z
dc.date.issued 2011-07
dc.identifier.citation Marine Biology, 2011, 158 (7), pp. 1667 - 1675
dc.identifier.issn 0025-3162
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/14911
dc.description.abstract We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i. e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples. © 2011 Springer-Verlag.
dc.language eng
dc.relation.isbasedon 10.1007/s00227-011-1663-1
dc.title Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging
dc.type Journal Article
dc.description.version Published
dc.parent Marine Biology
dc.journal.volume 7
dc.journal.volume 158
dc.journal.number 7 en_US
dc.publocation New York, USA en_US
dc.identifier.startpage 1667 en_US
dc.identifier.endpage 1675 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0602 Ecology
dc.personcode 107129
dc.percentage 100 en_US
dc.classification.name Ecology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - C3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history Closed (ID: 3)
utslib.collection.history Uncategorised (ID: 363)


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