Salt tolerance and screening for genetic changes in rice mutants after gamma irradiation using RAPD and microsatellite markers

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dc.contributor.author Hoang, T
dc.contributor.author De, FLF
dc.contributor.author Le, XT
dc.date.accessioned 2012-02-02T08:39:15Z
dc.date.issued 2009-01
dc.identifier.citation The Open Horticulture Journal, 2009, 2 (1), pp. 62 - 69
dc.identifier.issn 1874-8406
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/15022
dc.description.abstract Two Vietnamese rice mutants (M2-2 and M2-4 - second generation line 2 and line 4 respectively), induced by gamma irradiation were examined and compared to their parent (TT), and the Australian cultivar Amaroo (Am) for salt tolerance and genomic differences. Seedlings in tissue culture provided high yields and good quality DNA, especially with young shoots. Changes in DNA of the mutants in comparison to TT and Am were successfully assessed using RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) and RAMP-PCR (random amplified microsatellite polymorphism-polymerase chain reaction) molecular marker techniques. RAMP-PCR was better than RAPD-PCR at disclosing genetic changes between closely related individuals, and only RAMP-PCR was able to detect polymorphic bands between the two mutants. A measure of heterozygocity and genetic differentiation, the Gstatistic demonstrated that comparisons between Am and TT, M2-2 or M2-4 showed moderate genetic differentiation, but comparisons between TT and M2-2 or M2-4 showed low genetic differentiation. Percentage polymorphism between TT, M2-2, M2-4 and Am detected by RAPD-PCR were similar to those detected by RAMP-PCR (about 10%). DNA bands that were polymorphic between the two mutant lines were sequenced, two bands mapped on numerous chromosomes of rice, while two other bands mapped on one or two chromosomes, and these DNA bands on the whole coded for regulatory genes. The results showed that especially RAMP-PCR is a versatile, sensitive and cost effective method for measuring genomic differences between closely related plants
dc.publisher Bentham publishing
dc.title Salt tolerance and screening for genetic changes in rice mutants after gamma irradiation using RAPD and microsatellite markers
dc.type Journal Article
dc.parent The Open Horticulture Journal
dc.journal.volume 1
dc.journal.volume 2
dc.journal.number 1 en_US
dc.publocation India en_US
dc.identifier.startpage 62 en_US
dc.identifier.endpage 69 en_US
dc.cauo.name SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 0706 Horticultural Production
dc.personcode 860320
dc.percentage 100 en_US
dc.classification.name Horticultural Production en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.description.keywords NA
dc.description.keywords NA
dc.description.keywords NA
dc.description.keywords NA
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Faculty of Science/School of the Environment
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10


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