Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine cilia.

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Show simple item record Deutscher, AT Jenkins, C Minion, FC Seymour, LM Padula, MP Dixon, NE Walker, MJ Djordjevic, SP 2012-02-02T08:42:19Z 2010-10
dc.identifier.citation Molecular microbiology, 2010, 78 (2), pp. 444 - 458
dc.identifier.issn 0950-382X
dc.identifier.other C1 en_US
dc.description.abstract Mycoplasma hyopneumoniae, the causative agent of porcine enzootic pneumonia, adheres to ciliated respiratory epithelia resulting in ciliostasis and epithelial cell death. The cilium adhesin P97 (Mhp183) contains two repeat regions, designated R1 and R2, that play key roles in adherence. Eight pentapeptide repeats in R1 are sufficient to bind porcine cilia; however, both R1 and R2 are needed to bind heparin. Mhp271, a paralogue of P97, is the only other M. hyopneumoniae protein to contain both R1 and R2 repeats. These repeats are arranged as a set of three pentapeptide repeats (designated R1A₂₇₁), two decapeptide repeats (designated R2₂₇₁), and a second set of six pentapeptide repeats (designated R1B₂₇₁). To determine their function, recombinant proteins containing R1A₂₇₁) (F1₂₇₁) and R2₂₇₁-R1B₂₇₁ (F2₂₇₁) were constructed and used in in vitro binding assays. F2₂₇₁, but not F1₂₇₁, bound heparin (K(D)=8.1 ± 0.4 nM), fibronectin (K(D)=174 ± 13 nM) and porcine cilia. Pre-incubation of F2₂₇₁ with 100 µM heparin blocked cilium binding by ~69%. Cell surface shaving with trypsin combined with two-dimensional liquid chromatography coupled to tandem mass spectrometry analysis identified Mhp271 as surface-exposed. Our data suggest that both R1 and R2 in Mhp271 are involved in binding to host molecules.
dc.format Print-Electronic
dc.language eng
dc.relation.isbasedon 10.1111/j.1365-2958.2010.07345.x
dc.title Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine cilia.
dc.type Journal Article
dc.parent Molecular microbiology
dc.journal.volume 2
dc.journal.volume 78
dc.journal.number 2 en_US
dc.publocation UK en_US
dc.identifier.startpage 444 en_US
dc.identifier.endpage 458 en_US SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 0707 Veterinary Sciences
dc.personcode 970082
dc.personcode 107126
dc.personcode 117836
dc.percentage 100 en_US Veterinary Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords Animals
dc.description.keywords Cilia
dc.description.keywords Mycoplasma hyopneumoniae
dc.description.keywords DNA, Bacterial
dc.description.keywords Heparin
dc.description.keywords Fibronectins
dc.description.keywords Recombinant Proteins
dc.description.keywords Cloning, Molecular
dc.description.keywords Amino Acid Sequence
dc.description.keywords Proteomics
dc.description.keywords Molecular Sequence Data
dc.description.keywords Adhesins, Bacterial
dc.description.keywords Swine
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history Uncategorised (ID: 363)
utslib.collection.history Closed (ID: 3)

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