Regulation of nif gene expression and the energetics of N 2 fixation over the diel cycle in a hot spring microbial mat

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dc.contributor.author Steunou, A-S
dc.contributor.author Jensen, SI
dc.contributor.author Brecht, E
dc.contributor.author Becraft, ED
dc.contributor.author Bateson, MM
dc.contributor.author Kilian, O
dc.contributor.author Bhaya, D
dc.contributor.author Ward, DM
dc.contributor.author Peters, JW
dc.contributor.author Grossman, AR
dc.contributor.author Kühl, M
dc.date.accessioned 2012-02-02T10:57:42Z
dc.date.issued 2008-04
dc.identifier.citation ISME Journal, 2008, 2 (4), pp. 364 - 378
dc.identifier.issn 1751-7362
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/15468
dc.description.abstract Nitrogen fixation, a prokaryotic, O 2 -inhibited process that reduces N 2 gas to biomass, is of paramount importance in biogeochemical cycling of nitrogen. We analyzed the levels of nif transcripts of Synechococcus ecotypes, NifH subunit and nitrogenase activity over the diel cycle in the microbial mat of an alkaline hot spring in Yellowstone National Park. The results showed a rise in nif transcripts in the evening, with a subsequent decline over the course of the night. In contrast, immunological data demonstrated that the level of the NifH polypeptide remained stable during the night, and only declined when the mat became oxic in the morning. Nitrogenase activity was low throughout the night; however, it exhibited two peaks, a small one in the evening and a large one in the early morning, when light began to stimulate cyanobacterial photosynthetic activity, but O 2 consumption by respiration still exceeded the rate of O 2 evolution. Once the irradiance increased to the point at which the mat became oxic, the nitrogenase activity was strongly inhibited. Transcripts for proteins associated with energy-producing metabolisms in the cell also followed diel patterns, with fermentation-related transcripts accumulating at night, photosynthesis- and respiration-related transcripts accumulating during the day and late afternoon, respectively. These results are discussed with respect to the energetics and regulation of N 2 fixation in hot spring mats and factors that can markedly influence the extent of N 2 fixation over the diel cycle. © 2008 International Society for Microbial Ecology All rights reserved.
dc.language eng
dc.relation.isbasedon 10.1038/ismej.2007.117
dc.subject Gene expression, In situ, Microsensor, Nitrogen fixation, Synechococcus, Yellowstone national park, Synechococcus, microsensor, gene expression, nitrogen fixation, Yellowstone National Park, in situ, Synechococcus, Oxidoreductases, Bacterial Proteins, Reverse Transcriptase Polymerase Chain Reaction, Nitrogen Fixation, Ecosystem, Energy Metabolism, Oxygen Consumption, Transcription, Genetic, Gene Expression Regulation, Bacterial, Hot Springs, Photosynthesis, Light, Darkness, Bacterial Proteins, Darkness, Ecosystem, Energy Metabolism, Gene Expression Regulation, Bacterial, Hot Springs, Light, Nitrogen Fixation, Oxidoreductases, Oxygen Consumption, Photosynthesis, Reverse Transcriptase Polymerase Chain Reaction, Synechococcus, Transcription, Genetic, Synechococcus, microsensor, gene expression, nitrogen fixation, Yellowstone National Park, in situ, Microbiology
dc.subject Gene expression; In situ; Microsensor; Nitrogen fixation; Synechococcus; Yellowstone national park; Synechococcus; microsensor; gene expression; nitrogen fixation; Yellowstone National Park; in situ; Synechococcus; Oxidoreductases; Bacterial Proteins; Reverse Transcriptase Polymerase Chain Reaction; Nitrogen Fixation; Ecosystem; Energy Metabolism; Oxygen Consumption; Transcription, Genetic; Gene Expression Regulation, Bacterial; Hot Springs; Photosynthesis; Light; Darkness; Bacterial Proteins; Darkness; Ecosystem; Energy Metabolism; Gene Expression Regulation, Bacterial; Hot Springs; Light; Nitrogen Fixation; Oxidoreductases; Oxygen Consumption; Photosynthesis; Reverse Transcriptase Polymerase Chain Reaction; Synechococcus; Transcription, Genetic; Synechococcus, microsensor, gene expression, nitrogen fixation, Yellowstone National Park, in situ; Microbiology
dc.title Regulation of nif gene expression and the energetics of N 2 fixation over the diel cycle in a hot spring microbial mat
dc.type Journal Article
dc.description.version Published
dc.description.version Published
dc.description.version Published
dc.parent ISME Journal
dc.journal.volume 4
dc.journal.volume 2
dc.journal.number 4 en_US
dc.publocation United Kingdom en_US
dc.publocation USA
dc.identifier.startpage 364 en_US
dc.identifier.endpage 378 en_US
dc.cauo.name SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.conference American Society for Infomration Science and Technology
dc.for 1002 Environmental Biotechnology
dc.personcode 0000061230 en_US
dc.personcode 0000061187 en_US
dc.personcode 0000061231 en_US
dc.personcode 0000061232 en_US
dc.personcode 0000061233 en_US
dc.personcode 0000061234 en_US
dc.personcode 0000061235 en_US
dc.personcode 0000061236 en_US
dc.personcode 0000061237 en_US
dc.personcode 0000062169 en_US
dc.personcode 107129 en_US
dc.percentage 100 en_US
dc.classification.name Environmental Biotechnology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.date.activity 2010-10-22
dc.location.activity en_US
dc.location.activity Pittsburgh, USA
dc.description.keywords Synechococcus, microsensor, gene expression, nitrogen fixation, Yellowstone National Park, in situ en_US
dc.description.keywords NA
dc.description.keywords Synechococcus
dc.description.keywords Synechococcus
dc.description.keywords microsensor
dc.description.keywords microsensor
dc.description.keywords gene expression
dc.description.keywords gene expression
dc.description.keywords nitrogen fixation
dc.description.keywords nitrogen fixation
dc.description.keywords Yellowstone National Park
dc.description.keywords Yellowstone National Park
dc.description.keywords in situ
dc.description.keywords in situ
dc.description.keywords Synechococcus
dc.description.keywords microsensor
dc.description.keywords gene expression
dc.description.keywords nitrogen fixation
dc.description.keywords Yellowstone National Park
dc.description.keywords in situ
dc.description.keywords Gene expression
dc.description.keywords In situ
dc.description.keywords Microsensor
dc.description.keywords Nitrogen fixation
dc.description.keywords Synechococcus
dc.description.keywords Yellowstone national park
dc.description.keywords Gene expression
dc.description.keywords In situ
dc.description.keywords Microsensor
dc.description.keywords Nitrogen fixation
dc.description.keywords Synechococcus
dc.description.keywords Yellowstone national park
dc.staffid 107129 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - C3


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