Alterations in miRNA processing and expression in pleomorphic adenomas of the salivary gland.

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dc.contributor.author Zhang, X
dc.contributor.author Cairns, M
dc.contributor.author Rose, B
dc.contributor.author O'Brien, C
dc.contributor.author Shannon, K
dc.contributor.author Clark, J
dc.contributor.author Gamble, J
dc.contributor.author Tran, N
dc.date.accessioned 2012-02-02T10:58:14Z
dc.date.issued 2009-06
dc.identifier.citation International journal of cancer. Journal international du cancer, 2009, 124 (12), pp. 2855 - 2863
dc.identifier.issn 0020-7136
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/15486
dc.description.abstract Genome-wide microRNA (miRNA) expression profiling of salivary gland pleomorphic adenomas revealed a distinct expression signature consisting largely of upregulated miRNAs compared with matched normal tissue. Microarray data were confirmed by quantitative real time RT-PCR (q-RTPCR). Five miRNA genes upregulated in the tumours were found in close proximity to fragile sites and/or cancer associated genomic regions. Interestingly, q-RTPCR revealed an increase in the expression of components of the miRNA processing machinery (Dicer, Drosha, DGCR8 and p68) in tumours suggesting that the deregulation of miRNA expression may result from increased miRNA biogenesis. Target gene prediction analysis of the altered miRNAs indicated that genes in a number of signalling pathways important in tumourigenesis including WNT, MAPK and JAK-STAT were overrepresented. Significantly, the oncogene PLAG1 was overexpressed in our cohort and may be potentially regulated by these miRNAs. This is the first study to examine changes in the miRNA milieu in pleomorphic adenoma, the most common salivary gland tumour. This study has demonstrated an upregulation of both miRNAs genes and an upregulation of the miRNA processing machinery. These changes may be potential underlying mechanisms for the development of these benign tumours.
dc.format Print
dc.language eng
dc.relation.isbasedon 10.1002/ijc.24298
dc.subject Humans, Adenoma, Pleomorphic, Salivary Gland Neoplasms, Luciferases, MicroRNAs, 3' Untranslated Regions, DNA-Binding Proteins, Oligonucleotide Array Sequence Analysis, Tumor Markers, Biological, Gene Expression Profiling, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Gene Expression Regulation, Neoplastic, Chromosome Fragile Sites, 3' Untranslated Regions, Adenoma, Pleomorphic, Chromosome Fragile Sites, DNA-Binding Proteins, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Humans, Luciferases, MicroRNAs, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction, Salivary Gland Neoplasms, Signal Transduction, Tumor Markers, Biological, Tumor-Suppressor Gene, Microrna Genes, Transgenic Mice, Microarray Data, Small Rnas, Cancer, Plag1, Rearrangements, Carcinoma, Pathway, Oncology & Carcinogenesis
dc.subject Humans; Adenoma, Pleomorphic; Salivary Gland Neoplasms; Luciferases; MicroRNAs; 3' Untranslated Regions; DNA-Binding Proteins; Oligonucleotide Array Sequence Analysis; Tumor Markers, Biological; Gene Expression Profiling; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; Gene Expression Regulation, Neoplastic; Chromosome Fragile Sites; 3' Untranslated Regions; Adenoma, Pleomorphic; Chromosome Fragile Sites; DNA-Binding Proteins; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Luciferases; MicroRNAs; Oligonucleotide Array Sequence Analysis; Reverse Transcriptase Polymerase Chain Reaction; Salivary Gland Neoplasms; Signal Transduction; Tumor Markers, Biological; Tumor-Suppressor Gene; Microrna Genes; Transgenic Mice; Microarray Data; Small Rnas; Cancer; Plag1; Rearrangements; Carcinoma; Pathway; Oncology & Carcinogenesis
dc.title Alterations in miRNA processing and expression in pleomorphic adenomas of the salivary gland.
dc.type Journal Article
dc.parent International journal of cancer. Journal international du cancer
dc.journal.volume 12
dc.journal.volume 124
dc.journal.number 12 en_US
dc.publocation Hoboken en_US
dc.identifier.startpage 2855 en_US
dc.identifier.endpage 2863 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0601 Biochemistry and Cell Biology
dc.personcode 0000065419 en_US
dc.personcode 0000065420 en_US
dc.personcode 0000085735 en_US
dc.personcode 0000049057 en_US
dc.personcode 0000065421 en_US
dc.personcode 0000065414 en_US
dc.personcode 0000065422 en_US
dc.personcode 109012 en_US
dc.percentage 100 en_US
dc.classification.name Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:000265997500012 en_US
dc.location.activity United States
dc.description.keywords Tumor-Suppressor Gene; Microrna Genes; Transgenic Mice; Microarray Data; Small Rnas; Cancer; Plag1; Rearrangements; Carcinoma; Pathway en_US
dc.description.keywords Synethetic jets, flow control, boundary layer, laminar separation
dc.description.keywords 3' Untranslated Regions
dc.description.keywords Adenoma, Pleomorphic
dc.description.keywords Chromosome Fragile Sites
dc.description.keywords DNA-Binding Proteins
dc.description.keywords Gene Expression Profiling
dc.description.keywords Gene Expression Regulation, Neoplastic
dc.description.keywords Humans
dc.description.keywords Luciferases
dc.description.keywords MicroRNAs
dc.description.keywords Oligonucleotide Array Sequence Analysis
dc.description.keywords Reverse Transcriptase Polymerase Chain Reaction
dc.description.keywords Salivary Gland Neoplasms
dc.description.keywords Signal Transduction
dc.description.keywords Tumor Markers, Biological
dc.description.keywords Humans
dc.description.keywords Adenoma, Pleomorphic
dc.description.keywords Salivary Gland Neoplasms
dc.description.keywords Luciferases
dc.description.keywords DNA-Binding Proteins
dc.description.keywords MicroRNAs
dc.description.keywords 3' Untranslated Regions
dc.description.keywords Tumor Markers, Biological
dc.description.keywords Oligonucleotide Array Sequence Analysis
dc.description.keywords Gene Expression Profiling
dc.description.keywords Reverse Transcriptase Polymerase Chain Reaction
dc.description.keywords Signal Transduction
dc.description.keywords Gene Expression Regulation, Neoplastic
dc.description.keywords Chromosome Fragile Sites
dc.description.keywords Humans
dc.description.keywords Adenoma, Pleomorphic
dc.description.keywords Salivary Gland Neoplasms
dc.description.keywords Luciferases
dc.description.keywords DNA-Binding Proteins
dc.description.keywords MicroRNAs
dc.description.keywords 3' Untranslated Regions
dc.description.keywords Tumor Markers, Biological
dc.description.keywords Oligonucleotide Array Sequence Analysis
dc.description.keywords Gene Expression Profiling
dc.description.keywords Reverse Transcriptase Polymerase Chain Reaction
dc.description.keywords Signal Transduction
dc.description.keywords Gene Expression Regulation, Neoplastic
dc.description.keywords Chromosome Fragile Sites
dc.description.keywords Humans
dc.description.keywords Adenoma, Pleomorphic
dc.description.keywords Salivary Gland Neoplasms
dc.description.keywords Luciferases
dc.description.keywords MicroRNAs
dc.description.keywords 3' Untranslated Regions
dc.description.keywords DNA-Binding Proteins
dc.description.keywords Oligonucleotide Array Sequence Analysis
dc.description.keywords Tumor Markers, Biological
dc.description.keywords Gene Expression Profiling
dc.description.keywords Reverse Transcriptase Polymerase Chain Reaction
dc.description.keywords Signal Transduction
dc.description.keywords Gene Expression Regulation, Neoplastic
dc.description.keywords Chromosome Fragile Sites
dc.description.keywords Humans
dc.description.keywords Adenoma, Pleomorphic
dc.description.keywords Salivary Gland Neoplasms
dc.description.keywords Luciferases
dc.description.keywords MicroRNAs
dc.description.keywords 3' Untranslated Regions
dc.description.keywords DNA-Binding Proteins
dc.description.keywords Oligonucleotide Array Sequence Analysis
dc.description.keywords Tumor Markers, Biological
dc.description.keywords Gene Expression Profiling
dc.description.keywords Reverse Transcriptase Polymerase Chain Reaction
dc.description.keywords Signal Transduction
dc.description.keywords Gene Expression Regulation, Neoplastic
dc.description.keywords Chromosome Fragile Sites
dc.staffid 109012 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies


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