Kavalactones yangonin and methysticin induce apoptosis in human hepatocytes (HepG2) in vitro

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dc.contributor.author Tang, J
dc.contributor.author Dunlop, RA
dc.contributor.author Rowe, A
dc.contributor.author Rodgers, KJ
dc.contributor.author Ramzan, I
dc.date.accessioned 2012-02-02T11:01:06Z
dc.date.issued 2011-03
dc.identifier.citation Phytotherapy Research, 2011, 25 (3), pp. 417 - 423
dc.identifier.issn 0951-418X
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/15608
dc.description.abstract While cases of severe kava hepatotoxicity have been reported, studies examining the toxicity of individual kavalactones are limited. The present study examined the in vitro hepatotoxicity of kavain, methysticin and yangonin on human hepatocytes (HepG2) and the possible mechanism(s) involved. Cytotoxicity was assessed using lactate dehydrogenase (LDH) and ethidium bromide (EB) assays. The mode of cell death was analysed with acridine orange/ethidium bromide dual staining with fluorescence microscopy. Glutathione oxidation was measured using the ortho-phthalaldehyde (OPT) fluorescence assay. Kavain had minimal cytotoxicity, methysticin showed moderate concentration-dependent toxicity and yangonin displayed marked toxicity with ∼40% reduction in viability in the EB assay. Acridine orange/ethidium bromide staining showed the predominant mode of cell death was apoptosis rather than necrosis. No significant changes were observed in glutathione levels, excluding this as the primary mechanism of cell death in this model. Further studies may elucidate the precise apoptotic pathways responsible and whether toxic kavalactone metabolites are involved. Copyright © 2010 John Wiley & Sons, Ltd.
dc.language eng
dc.relation.isbasedon 10.1002/ptr.3283
dc.title Kavalactones yangonin and methysticin induce apoptosis in human hepatocytes (HepG2) in vitro
dc.type Journal Article
dc.description.version Published
dc.description.version Published
dc.description.version Published
dc.description.version Published
dc.description.version Published
dc.parent Phytotherapy Research
dc.journal.volume 3
dc.journal.volume 25
dc.journal.number 3 en_US
dc.publocation United States en_US
dc.identifier.startpage 417 en_US
dc.identifier.endpage 423 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 1115 Pharmacology and Pharmaceutical Sciences
dc.personcode 111642
dc.personcode 115899
dc.percentage 100 en_US
dc.classification.name Pharmacology and Pharmaceutical Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords cytotoxicity;kavalactone;kavain;methysticin;yangonin;apoptosis;HepG2;hepatocyte en_US
dc.description.keywords P-glycoprotein
dc.description.keywords endocytosis
dc.description.keywords exocytosis
dc.description.keywords Rab5
dc.description.keywords RalA
dc.description.keywords multidrug resistance in cancer
dc.description.keywords cytotoxicity
dc.description.keywords cytotoxicity
dc.description.keywords cytotoxicity
dc.description.keywords kavalactone
dc.description.keywords kavalactone
dc.description.keywords kavalactone
dc.description.keywords kavalactone
dc.description.keywords kavain
dc.description.keywords kavain
dc.description.keywords kavain
dc.description.keywords kavain
dc.description.keywords methysticin
dc.description.keywords methysticin
dc.description.keywords methysticin
dc.description.keywords methysticin
dc.description.keywords yangonin
dc.description.keywords yangonin
dc.description.keywords yangonin
dc.description.keywords yangonin
dc.description.keywords apoptosis
dc.description.keywords apoptosis
dc.description.keywords apoptosis
dc.description.keywords apoptosis
dc.description.keywords HepG2
dc.description.keywords HepG2
dc.description.keywords HepG2
dc.description.keywords HepG2
dc.description.keywords hepatocyte
dc.description.keywords hepatocyte
dc.description.keywords hepatocyte
dc.description.keywords hepatocyte
dc.description.keywords cytotoxicity
dc.description.keywords cytotoxicity
dc.description.keywords kavalactone
dc.description.keywords kavalactone
dc.description.keywords kavain
dc.description.keywords kavain
dc.description.keywords methysticin
dc.description.keywords methysticin
dc.description.keywords yangonin
dc.description.keywords yangonin
dc.description.keywords apoptosis
dc.description.keywords apoptosis
dc.description.keywords HepG2
dc.description.keywords HepG2
dc.description.keywords hepatocyte
dc.description.keywords hepatocyte
dc.description.keywords Apoptosis
dc.description.keywords Cytotoxicity
dc.description.keywords Hepatocyte
dc.description.keywords HepG2
dc.description.keywords Kavain
dc.description.keywords Kavalactone
dc.description.keywords Methysticin
dc.description.keywords Yangonin
dc.description.keywords Apoptosis
dc.description.keywords Cytotoxicity
dc.description.keywords Hepatocyte
dc.description.keywords HepG2
dc.description.keywords Kavain
dc.description.keywords Kavalactone
dc.description.keywords Methysticin
dc.description.keywords Yangonin
dc.description.keywords Apoptosis
dc.description.keywords Cytotoxicity
dc.description.keywords Hepatocyte
dc.description.keywords HepG2
dc.description.keywords Kavain
dc.description.keywords Kavalactone
dc.description.keywords Methysticin
dc.description.keywords Yangonin
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Faculty of Science/School of Medical and Molecular Sciences
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies


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