Difficult proteins.

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dc.contributor.author Herbert, B
dc.contributor.author Harry, E
dc.date.accessioned 2012-02-02T11:12:45Z
dc.date.issued 2009
dc.identifier.citation 2009, 519 pp. 47 - 63
dc.identifier.other B2 en_US
dc.identifier.uri http://hdl.handle.net/10453/16739
dc.description.abstract The degree of protein diversity and dynamic range within organisms means that even the simplest proteome cannot be captured by any single extraction and separation step. New techniques have focused on major protein classes often under-represented in proteome analysis; low abundance, membrane, and alkaline proteins. The last decade has seen considerable technology development in fractionation tools aimed at complexity reduction in many forms. The key outcome of complexity reduction is that each fraction, or sub-proteome, can be studied in more detail, and proteins which would have remained undetected in a total extract are present in sufficient quantities. However, the tools available are fractionations, not amplifications, and like all mining for rare and difficult items, a large amount of starting material is often required. The key shortcomings of many proteome analysis techniques are now well documented. With this knowledge, the best modern proteomics 'platform' involves combining multiple protein extractions, gel and chromatographic separations, and multiple MS analysis methods.
dc.relation.isbasedon 10.1007/978-1-59745-281-6_4
dc.title Difficult proteins.
dc.type Chapter
dc.journal.volume 519
dc.journal.number en_US
dc.publocation New York en_US
dc.identifier.startpage 47 en_US
dc.identifier.endpage 63 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 110106 Medical Biochemistry: Proteins and Peptides (Incl. Medical Proteomics)
dc.for 060504 Microbial Ecology
dc.for 060501 Bacteriology
dc.personcode 995003
dc.personcode 997815
dc.percentage 40 en_US
dc.classification.name Bacteriology en_US
dc.classification.type FOR-08 en_US
dc.edition 1 en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)


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