P2X7 receptor-mediated killing of an intracellular parasite, Toxoplasma gondii, by human and murine macrophages

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dc.contributor.author Lees, MP
dc.contributor.author Lees, MP
dc.contributor.author Fuller, S
dc.contributor.author Fuller, S
dc.contributor.author McLeod, R
dc.contributor.author McLeod, R
dc.contributor.author Boulter, N
dc.contributor.author Boulter, N
dc.contributor.author Miller, CM
dc.contributor.author Miller, CM
dc.contributor.author Zakrzewski, AM
dc.contributor.author Zakrzewski, AM
dc.contributor.author Mui, E
dc.contributor.author Mui, E
dc.contributor.author Witola, WH
dc.contributor.author Witola, WH
dc.contributor.author Coyne, J
dc.contributor.author Coyne, J
dc.contributor.author Hargrave, A
dc.contributor.author Hargrave, A
dc.contributor.author Jamieson, S
dc.contributor.author Jamieson, S
dc.contributor.author Blackwell, J
dc.contributor.author Blackwell, J
dc.contributor.author Wiley, JS
dc.contributor.author Wiley, JS
dc.contributor.author Smith, NC
dc.contributor.author Smith, NC
dc.date.accessioned 2012-02-10T06:09:48Z
dc.date.issued 2011-01
dc.identifier.citation Journal of Immunology, 2011, 184 (12), pp. 7040 - 7046
dc.identifier.citation Journal of Immunology, 2011, 184 (12), pp. 7040 - 7046
dc.identifier.issn 0022-1767
dc.identifier.issn 0022-1767
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/17140
dc.description.abstract The P2X7R is highly expressed on the macrophage cell surface, and activation of infected cells by extracellular ATP has been shown to kill intracellular bacteria and parasites. Furthermore, single nucleotide polymorphisms that decrease receptor function reduce the ability of human macrophages to kill Mycobacterium tuberculosis and are associated with extrapulmonary tuberculosis. In this study, we show that macrophages from people with the 1513C (rs3751143, NM-002562.4:c.1487A>C) loss-of-function P2X 7R single nucleotide polymorphism are less effective in killing intracellular Toxoplasma gondii after exposure to ATP compared with macrophages from people with the 1513A wild-type allele. Supporting a P2X 7R-specific effect on T. gondii, macrophages from P2X7R knockout mice (P2X7R-/-) are unable to kill T. gondii as effectively as macrophages from wild-type mice. We show that P2X 7R-mediated T. gondii killing occurs in parallel with host cell apoptosis and is independent of NO production
dc.description.abstract The P2X7R is highly expressed on the macrophage cell surface, and activation of infected cells by extracellular ATP has been shown to kill intracellular bacteria and parasites. Furthermore, single nucleotide polymorphisms that decrease receptor function reduce the ability of human macrophages to kill Mycobacterium tuberculosis and are associated with extrapulmonary tuberculosis. In this study, we show that macrophages from people with the 1513C (rs3751143, NM-002562.4:c.1487A>C) loss-of-function P2X 7R single nucleotide polymorphism are less effective in killing intracellular Toxoplasma gondii after exposure to ATP compared with macrophages from people with the 1513A wild-type allele. Supporting a P2X 7R-specific effect on T. gondii, macrophages from P2X7R knockout mice (P2X7R-/-) are unable to kill T. gondii as effectively as macrophages from wild-type mice. We show that P2X 7R-mediated T. gondii killing occurs in parallel with host cell apoptosis and is independent of NO production
dc.publisher American Association of Immunologists
dc.publisher American Association of Immunologists
dc.relation.isbasedon 10.4049/jimmunol.1000012
dc.relation.isbasedon 10.4049/jimmunol.1000012
dc.subject adenosine triphosphate, purinergic P2X7 receptor, nitric oxide, purinergic P2 receptor, Immunology
dc.subject adenosine triphosphate, purinergic P2X7 receptor, nitric oxide, purinergic P2 receptor, Immunology
dc.subject adenosine triphosphate; purinergic P2X7 receptor; nitric oxide; purinergic P2 receptor; Immunology
dc.subject adenosine triphosphate; purinergic P2X7 receptor; nitric oxide; purinergic P2 receptor; Immunology
dc.title P2X7 receptor-mediated killing of an intracellular parasite, Toxoplasma gondii, by human and murine macrophages
dc.title P2X7 receptor-mediated killing of an intracellular parasite, Toxoplasma gondii, by human and murine macrophages
dc.type Journal Article
dc.parent Journal of Immunology
dc.parent Journal of Immunology
dc.journal.volume 12
dc.journal.volume 12
dc.journal.volume 184
dc.journal.volume 184
dc.journal.number 12 en_US
dc.publocation USA en_US
dc.identifier.startpage 7040 en_US
dc.identifier.endpage 7046 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 1107 Immunology
dc.for 1107 Immunology
dc.personcode 044579 en_US
dc.personcode 0000020556 en_US
dc.personcode 0000067922 en_US
dc.personcode 011172 en_US
dc.personcode 970043 en_US
dc.personcode 100992 en_US
dc.personcode 0000067595 en_US
dc.personcode 0000072838 en_US
dc.personcode 0000067602 en_US
dc.personcode 0000067593 en_US
dc.personcode 0000061837 en_US
dc.personcode 0000060219 en_US
dc.personcode 0000020554 en_US
dc.personcode 960087 en_US
dc.percentage 100 en_US
dc.classification.name Immunology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords adenosine triphosphate; purinergic P2X7 receptor; nitric oxide; purinergic P2 receptor en_US
dc.description.keywords High-temperature superconductors (HTSs), linear synchronous motor, machine control, pulse magnetization, space vector pulsewidth modulation (SVPWM), thrust and normal force, yttrium barium copper oxide (YBCO) bulk.
dc.description.keywords adenosine triphosphate
dc.description.keywords adenosine triphosphate
dc.description.keywords purinergic P2X7 receptor
dc.description.keywords purinergic P2X7 receptor
dc.description.keywords nitric oxide
dc.description.keywords nitric oxide
dc.description.keywords purinergic P2 receptor
dc.description.keywords purinergic P2 receptor
dc.staffid 960087 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Faculty of Science


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